[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Submit :: Contact Us ::
:: Volume 7, Issue 4 (winter 2018) ::
J Fasa Univ Med Sci 2018, 7(4): 428-437 Back to browse issues page
An Overview of the Second and Third-Generation of DNA Sequencing Technologies
Fatemeh Ghorbani Parsa 1, Mohammad Ali Hossein Pour Feizi 2
1- Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2- Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran , pourfeizi@eastp.ir
Abstract:   (1248 Views)

DNA sequence determination is a tremendous human achievement. DNA sequencing includes several methods and technologies in use for determining the order of the nucleotide bases in a molecule of nucleic acid. Knowledge of DNA sequences has become indispensable for the basic biological researches. Nucleotides sequence determination is used in numerous applied fields such as diagnostics, biotechnology, forensic biology and biological systematics. The advent of DNA sequencing has significantly accelerated biological researches and discoveries.
There are several generations of DNA sequencing technologies that can be well characterized through their nature and the kind of output they provide. Dideoxy terminator sequencing developed by Sanger dominated for 30 years and was the workhorse used for the Human Genome Project. In 2005 the first 2nd generation sequencer was presented with an output orders of magnitude higher than Sanger sequencing and dramatically decreased cost. We are now at the dawn of the 3rd generation of sequencing systems.
Researchers in the field of genetics in Iran use this technology in their studies, but unfortunately our literature lacks proper Persian language resources. This review briefly describes the current high-throughput nucleotide sequencing platforms commercially available.

Keywords: DNA sequencing, High Throughput Nucleotide Sequencing, second generation sequencing, third generation sequencing
Full-Text [PDF 831 kb]   (406 Downloads)    
Type of Study: Review | Subject: Genetics
Received: 2017/01/22 | Accepted: 2017/07/18 | Published: 2018/01/7
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghorbani Parsa F, Hossein Pour Feizi M A. An Overview of the Second and Third-Generation of DNA Sequencing Technologies. J Fasa Univ Med Sci. 2018; 7 (4) :428-437
URL: http://journal.fums.ac.ir/article-1-1262-en.html


Volume 7, Issue 4 (winter 2018) Back to browse issues page
Journal of Fasa University of Medical Sciences
Persian site map - English site map - Created in 0.07 seconds with 30 queries by YEKTAWEB 3701