Journal of Advanced Biomedical Sciences
JABS
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Volume 3, Issue 2 (9-2013)
JABS 2013, 3(2): 117-123 |
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javanmardi K, soltanihekmat A, shekoohi M, hasanein P, bakhshi M, ghodsi R, et al . The Role of Parabrachial GABAA Receptors in Pain Modulation in Rats. JABS 2013; 3 (2) :117-123
URL: http://jabs.fums.ac.ir/article-1-282-en.html
URL: http://jabs.fums.ac.ir/article-1-282-en.html
Kazem Javanmardi1 
, Ava Soltanihekmat * 2, Masoomeh Shekoohi3 , Parisa Hasanein4 , Mehdi Bakhshi5 , Roghayeh Ghodsi1 , Leila Rezaeian6 , Akhtar Bordbar1
, Ava Soltanihekmat * 2, Masoomeh Shekoohi3 , Parisa Hasanein4 , Mehdi Bakhshi5 , Roghayeh Ghodsi1 , Leila Rezaeian6 , Akhtar Bordbar1
1- Department of physiology, Fasa University of Medical Sciences, Fasa, Iran
2- Department of physiology, Fasa University of Medical Sciences, Fasa, Iran , Soltanihekmat@yahoo.com
3- Department of physiology, Jahrom Branch,, Islamic Azad University, Jahrom, Iran
4- Department of Biology, Buali Sina University, Hamedan, Iran
5- Department of Pathology, Fasa University of Medical Sciences, Fasa, Iran
6- Farhangian University. Shiraz. Iran.
2- Department of physiology, Fasa University of Medical Sciences, Fasa, Iran , Soltanihekmat@yahoo.com
3- Department of physiology, Jahrom Branch,, Islamic Azad University, Jahrom, Iran
4- Department of Biology, Buali Sina University, Hamedan, Iran
5- Department of Pathology, Fasa University of Medical Sciences, Fasa, Iran
6- Farhangian University. Shiraz. Iran.
Abstract: (24473 Views)
Background & Objective: The parabrachial nucleus is a critical link in the transmission of short latency nociceptive information to midbrain neurons. GABA(A) receptors have bidirectional roles in controlling nociception and are abundant in the parabrachial region . We examined the effects of bilateral intra parabrachial microinjection of different doses of the GABA(A) receptor agonist, muscimol, and the GABA(A) receptor antagonist, bicuculline, on pain modulation using a tail-flick test
.
Materials & Methods: Rats were anaesthetized with sodium pentobarbital (55 mg/kg) and then special cannulas were inserted stereotaxically into the parabrachial nucleus. After 1 week of recovery, the effects of microinjection of muscimol, (62.5, 125,250 ng/side) or bicuculline, (50,100,200 ng/side) into the parabrachial on tail flick latencies were assessed. Tail-flick latencies were measured for 60 minutes every 5 min after drug microinjection.
Results: Microinjection of muscimol (62.5, 125 ng/side) and bicuculline (50,100,200 ng/side) into the parabrachial did not have any statistically significant effect on tail-flick latency. Administration of, muscimol, (250 ng/side) produced thermal hyperalgesia (P<0.05).
Conclusion: The results of the present study showed that in this model of pain gaba a receptors in the paracrachial region are not Endogenously activated but these receptors in this region have a potential to affect pain modulation.
Materials & Methods: Rats were anaesthetized with sodium pentobarbital (55 mg/kg) and then special cannulas were inserted stereotaxically into the parabrachial nucleus. After 1 week of recovery, the effects of microinjection of muscimol, (62.5, 125,250 ng/side) or bicuculline, (50,100,200 ng/side) into the parabrachial on tail flick latencies were assessed. Tail-flick latencies were measured for 60 minutes every 5 min after drug microinjection.
Results: Microinjection of muscimol (62.5, 125 ng/side) and bicuculline (50,100,200 ng/side) into the parabrachial did not have any statistically significant effect on tail-flick latency. Administration of, muscimol, (250 ng/side) produced thermal hyperalgesia (P<0.05).
Conclusion: The results of the present study showed that in this model of pain gaba a receptors in the paracrachial region are not Endogenously activated but these receptors in this region have a potential to affect pain modulation.
Type of Study: Research |
Subject:
Physiology
Received: 2013/07/22 | Accepted: 2013/10/6 | Published: 2013/10/6
Received: 2013/07/22 | Accepted: 2013/10/6 | Published: 2013/10/6
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This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)