:: Volume 7, Number 2 (Summer 2017) ::
J Fasa Univ Med Sci 2017, 7(2): 199-209 Back to browse issues page
Comparing the effects of fetal bovine and human sera on isolation, expansion and osteogenic differentiation of adipose-derived stem cells
Hossein Mohammadpour1, Vahid Razban2, Dr Mahdi Mahmoodi3, Mohammad Reza Hajizadeh4, Maryam Hosseinipour1, Atena Sadat Ghoreishi1, Gity Farsi1, Farzaneh Sadat Hosseini1, Ali Reza Khoshdel 5
1- Dept. of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2- Dept of Molecular Medicine, Faculty of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
3- Molecular Medicine Research Center and Dept. of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
4- Dept of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
5- Dept. of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran , Alireza.khoshdel@gmail.com
Abstract:   (168 Views)

Background & Objectives: Adipose-derived stem cells (ADSCs) have been investigated as a promising cell source for therapeutic and engineering applications. Common proliferation protocols use fetal bovine serum (FBS) as a growth factor which is potentially a source of pathogens, and contains animal antigens that can cause an allergic reaction after transplantation in the recipient, and regarding immunity, human serum (HS) could be a suitable alternative to it. The aim of this study is to compare the culture medium enriched with FBS or HS as supplements in the proliferation and differentiation of ADSCs.
Materials & Methods: Human serum was extracted from 90 ml venous blood of a healthy person, taken in respective intervals. The ADSCs were isolated according to the protocol from adipose tissue after lipolysis operation and cultured by mediums enriched with FBS or HS. Expression of the surface markers of ADSCs was investigated by flow cytometry. Osteogenic differentiation was evaluated by Alizarin Red staining method.
Results: The analysis of cell growth showed that the isolation and proliferation of ADSCs in both media (HS & FBS) were similar, but there was a significant difference in case of differentiation in the HS medium.
Conclusion: This study showed that FBS could be replaced by HS in case of isolation, proliferation and differentiation of stem cells studies as supplement. Furthermore, our data suggest a fast and safe proliferation protocol by using human serum in the stem cells culture and cell-based therapies.
 

Keywords: adipose-derived stem cells (ADSCs), human serum (HS), fetal bovine serum (FBS)
Full-Text [PDF 1290 kb]   (24 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2016/10/11 | Accepted: 2017/02/10 | Published: 2017/09/3



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Volume 7, Number 2 (Summer 2017) Back to browse issues page