Volume 7, Issue 1 (5-2017)                   JABS 2017, 7(1): 128-133 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohammadi J, Amini K. Detection of virulence genes in Uropathogenic E. coli (UPEC) strains by Multiplex-PCR method. JABS 2017; 7 (1) :128-133
URL: http://jabs.fums.ac.ir/article-1-1042-en.html
1- , Saveh Branch, Islamic Azad University, Saveh, Iran.
2- Saveh Branch, Islamic Azad University, Saveh, I.R, Iran , dr_kumarss_amini@yahoo.com
Abstract:   (8799 Views)

Background & Objectives: Urinary tract infection caused by E. coli is one of the most common illnesses in all age groups worldwide. Presence of virulence genes is a key factor in bacterial pathogens in uroepithelial cells. The present study was performed to detect iha, iroN, ompT genes in the Uropathogenic E.coli isolates from clinical samples using multiplex-PCR method in Kerman.

Materials & Methods: In this descriptive cross-sectional study, 200 samples of patients with urinary tract infections in Kerman hospitals were collected. After biochemical and microbiological tests, all strains were tested with regard to the presence of iha, iroN, and ompT genes using multiplex-PCR method.

Results: The results of Multiplex-PCR showed that all specimens had one, two, or three virulence genes simultaneously. The highest and lowest frequency distribution of genes was related to iha (56.7%) and iroN (20%) respectively.

Conclusion: According to the prevalence of urinary tract infection in the community and distribution of resistance and virulence factors, the fast and accurate detection of the strains and virulence genes is necessary

Keywords: UPEC, iha, iroN, ompT, multiplex-PCR
Full-Text [PDF 1134 kb]   (4044 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2016/04/25 | Accepted: 2016/09/30 | Published: 2017/06/7

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)