:: Volume 7, Number 2 (Summer 2017) ::
J Fasa Univ Med Sci 2017, 7(2): 210-216 Back to browse issues page
Molecular analysis of various classes of integrons in the clinical Pseudomonas aeruginosa isolates by Multiplex-PCR
Atousa Rashidi1, Gholamali Moradli 2
1- msc Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
2- assistance profesor Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran. , moradli.mic@gmail.com
Abstract:   (169 Views)

Background & Objective: The presence of classes I, II and III Integrons in multidrug-resistant Pseudomonas aeruginosa strains, has created new concerns in clinics. This study aimed to investigate the prevalence of class I, II and III Integron in clinical Pseudomonas aeruginosa isolates.
Materials & Methods: In the cross-sectional study, 60 isolates of P. aeruginosa were obtained from the microbial-Laboratory of Rasool Akram Hospital in Tehran. Antibiotic susceptibility test was performed by the agar diffusion method based on CLSI guidelines on the Mueller Hinton agar medium. The genomic DNA was extracted using DNA extraction kit and M-PCR was performed for amplification of intI, intII and intIII genes.
Results: The highest resistance rate was in the strains that harbored integron, so that 96.6% of strains were resistant to amoxicillin. The results showed that all 60 strains had resistance (100%) to amikacin. The highest frequency was related to intI (40%) and the lowest frequency was related to intIII genes (16.6%).
Conclusion: Considering the high prevalence of class I Integron in all Pseudomonas aeruginosa-resistant isolates and its relation with different drug resistance patterns, the right solution to infection control and treatment in hospitals is essential to prevent further spread of it.
 

Keywords: Class I, II, III Integron genes, Pseudomonas aeruginosa, Multiplex-PCR.
Full-Text [PDF 1390 kb]   (17 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2016/08/7 | Accepted: 2017/01/28 | Published: 2017/09/3



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Volume 7, Number 2 (Summer 2017) Back to browse issues page