:: Volume 7, Issue 4 (winter 2018) ::
J Fasa Univ Med Sci 2018, 7(4): 573-581 Back to browse issues page
Investigating the expression of CCAT2 gene as a new molecular marker in breast tumors
Samira Kazemzadeh1, Esmaeil Babaei 2, Mohammad ali Hoseinpour fizi1
1- Department of Biology, Faculty of Natural Sciences, Tabriz University
2- Department of Biology, Faculty of Natural Sciences, Tabriz University , babaei@tabrizu.ac.ir
Abstract:   (3091 Views)

Background & Objectives: lnc-CCAT2 is a non-protein coding RNA, which is highly expressed in tissues and cancer cell types. Due to the increasing incidence of breast tumors in recent years, in this research, the qualification of CCAT2 gene expression is assessed as a potential molecular marker in the diagnosis and treatment of breast tumors.
Material & Methods: Totally, 35 breast tumor specimens and 35 non-tumoral ones related to the tumor margins were studied using quantitative real-time PCR method. Gene β2m was used as the internal control. Obtained results were statistically analyzed Using the SPSS and sigmaplot software.
Results: The results showed that CCAT2 gene expression, significantly increased in breast tumor samples compared to marginal non-tumor specimens. (P < 0.01)
Conclusion: This results showed that CCAT2 gene can be applied as a diagnostic marker for the identification and diagnosis of breast tumor tissues and non-tumor types. Therefore, it can be used alongside other conventional Diagnostic Laboratorial methods. More studies are underway on the biomarking potentiality of this gene.

Keywords: Breast cancer, Non-coding RNA, Real-time PCR, CCAT2
Full-Text [PDF 485 kb]   (458 Downloads)    
Type of Study: Research | Subject: Medical Genetics
Received: 2016/12/24 | Accepted: 2017/05/19 | Published: 2017/11/12


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Volume 7, Issue 4 (winter 2018) Back to browse issues page