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:: Volume 7, Issue 4 (winter 2018) ::
J Fasa Univ Med Sci 2018, 7(4): 511-520 Back to browse issues page
The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats
Seyed Damoun Sadoughi 1, Mohammad Amin Edalatmanesh 2, Raheleh Rahbarian 3
1- Department of Biology, College of Science, Fars Science and Research Branch, Islamic Azad University, Fars, Iran.Department of Biology, College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Department of Biology, College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran , amin.edalatmanesh@gmail.com
3- Department of Biology, College of Sciences, Payam-e-Noor University, Tehran, Iran
Abstract:   (1301 Views)

Background & Objective: Diabetes causes reproductive disorders by endocrine glands dysfunction. Also curcumin has antioxidant and hypoglycemic properties. The purpose of this study was to determine the effect of curcumin on pituitary-gonadal axis, DNA oxidative damage and antioxidant enzymes activity of testicular tissue in male type one diabetic rats.
Material & Methods: In this experimental study 40 male Wistar rats were allocated into 5 equal groups (n=8). Control group, non-treated diabetic group (30 days, intraperitoneal injection of dimethyl sulfoxide) and treated diabetic groups with doses 50, 100 and 150 mg/kg of curcumin (30 days, intraperitoneal injection of curcumin dissolved in dimethyl sulfoxide). Diabetes was induced using an intraperitoneal injection of 240 mg/kg alloxan. At the end of treatments, serum levels of LH, FSH, estrogen, testosterone, dihydrotestosterone and levels of SOD, CAT, GPX, HOdG-8 and MDA in testicle tissue were measured by ELISA. The statistical analysis was carried out using one-way ANOVA and post-hoc Tukey tests (p<0.05).
Result: Compared to the non-treated diabetic group, administration of curcumin with doses of 100 and 150 mg/kg caused dose-dependent significant increased serum levels of LH, FSH, estrogen, testosterone and dihydrotestosterone, dose-dependent significant decreased HOdG-8 and MDA and also dose-dependent significant increased enzyme of SOD, CAT, and GPX in diabetic rats’ testicle (p<0.05).
Conclusion: Administration of curcumin increases pituitary-gonadal axis activity, decreases lipid peroxidation and DNA oxidative damage and also increases antioxidant enzyme activity of testicular tissue in diabetic rats.
 

Keywords: Diabetes Mellitus, Curcumin, Testis, Antioxidant Enzymes, Rat
Full-Text [PDF 333 kb]   (245 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2017/01/20 | Accepted: 2017/04/21 | Published: 2018/01/7
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Sadoughi S D, Edalatmanesh M A, Rahbarian R. The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats. J Fasa Univ Med Sci. 2018; 7 (4) :511-520
URL: http://journal.fums.ac.ir/article-1-1287-en.html


Volume 7, Issue 4 (winter 2018) Back to browse issues page
Journal of Fasa University of Medical Sciences
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