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:: Volume 7, Number 1 (Spring 2017) ::
J Fasa Univ Med Sci 2017, 7(1): 39-52 Back to browse issues page
Phytosynthesis of Silver Nanoparticles using aqueous extract of Camellia sinensis L. and study of their antibacterial activities
Masoumeh Etemadi1, Davod Mohebbi-Kalhori 2, Omid Azizian Shermeh3, Ali Qasemi4
1- Chemical Engineering Department, Shahid Nikbakht Faculty of Engineering, University of Sistan and Baluchestan, Zahedan, Iran
2- Assistant professor Chemical Engineering Department, Shahid Nikbakht Faculty of Engineering, University of Sistan and Baluchestan, Zahedan, Iran , davoodmk@eng.usb.ac.ir
3- Department of Chemistry, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
4- Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
Abstract:   (1240 Views)

Background and Objectives: Nowadays, the use of silver nanoparticles (AgNPs) as a substance with effective antibacterial activity and biosynthesis has been increased because of being low cast and compatible with the environment. This study reports biosynthesis AgNPs with aqueous extract of GT (C.sinensis) and compares antibacterial activity with aqueous, methanol and ethanol extract.

Methods: To prepare nanoparticle, 2 ml extract was added to 4ml silver nitrate solution (AgNO3) with concentration 1Mm. Effective parameter on synthesis AgNPs were optimized and studied to obtain individual shape and size of nanoparticles. For characterization AgNPs, transmission electron microscopy and X-ray diffractometry were applied.  Disk diffusion, well diffusion and Minimum inhibition concentration (MIC) for the investigation of antimicrobial activity of the extracts and AgNPs against Staphylococcus aureus and Escherichia coli were utilized respectively.

Results: AgNPs showed maximum absorbance at 411 nm and they were spherical shape with the average size between 14.52-18.23 nm. AgNPs high antibacterial activity was compared with three extracts against microorganism.

Conclusion: AgNPs which were synthesized by GT have very good antibacterial activity compared extracts and can be used as an effective alternative in medical application.

Keywords: Camellia sinensis, Phytosynthesis, silver nanoparticles, Antibacterial activity
Full-Text [PDF 2009 kb]   (393 Downloads)    
Type of Study: Research | Subject: Nanomedicine
Received: 2016/12/13 | Accepted: 2017/01/22 | Published: 2017/06/6
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Etemadi M, Mohebbi-Kalhori D, Azizian Shermeh O, Qasemi A. Phytosynthesis of Silver Nanoparticles using aqueous extract of Camellia sinensis L. and study of their antibacterial activities. J Fasa Univ Med Sci. 2017; 7 (1) :39-52
URL: http://journal.fums.ac.ir/article-1-1305-en.html
Volume 7, Number 1 (Spring 2017) Back to browse issues page
Journal of Fasa University of Medical Sciences
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