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:: Volume 8, Issue 1 (Spring 2018) ::
J Fasa Univ Med Sci 2018, 8(1): 628-636 Back to browse issues page
Investigating CpG islands methylation in P16/Ink4 gene promoter regions and the effect of Interleukin-17 gene polymorphism on this methylation in patients with breast cancer
Sirous Naeimi
Department of Genetics, Colleague of science, Kazerun branch,Islamic Azad University,Kazerun, Iran , naeimis@kau.ac.ir
Abstract:   (1933 Views)

Background & objective: Studies have shown that increased methylation of CpG islands is one of the important mechanisms in gene silencing. Protein P16 / Ink4 plays an important role in the negative regulator of cell cycle process. Inflammation, including factors that affect gene methylation and IL-17, as an inflammatory cytokine, can play a role in this case. This cytokine gene has several polymorphisms which are involved in the expression of it. According to the statement, the purpose of this study is to investigate IL-17 gene polymorphism on gene promoter methylation of P16 / Ink4 and its relation to breast cancer diseases.
 
Material & Methods: In this case - control study, a total of 40 Women with Breast cancer and 40 healthy women on September 2015 were examined. DNA was extracted and for gene promoter methylation, MSPCR method was used. Single nucleotide Polymorphisms of the IL-17 gene were analyzed by the PCR-RFLP method. Data were compared in both groups by using Pearson’s chi-square and Hardy-Weinberg equilibrium test.
Results: Results confirm the fact that, there is a relationship between P16/Ink4 gene promoter methylation and breast cancer disease So that, the promoter of P16/Ink4 gene in healthy individuals was much more unmethylated than patients (p<0.05). On the other hand there is no significant difference between IL-17 gene polymorphisms and DAP-kinase gene methylation (P>0.05).
Conclusion: It seems that increases of P16/Ink4 gene promoter unmethylation in control subjects is associated with the likelihood of being resistant to breast cancer.

Keywords: Polymorphism, Breast Cancer, IL-17, Methylation, P16/Ink4
Full-Text [PDF 1047 kb]   (474 Downloads)    
Type of Study: Research | Subject: Medical Genetics
Received: 2017/04/16 | Accepted: 2017/11/1 | Published: 2018/05/23
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Naeimi S. Investigating CpG islands methylation in P16/Ink4 gene promoter regions and the effect of Interleukin-17 gene polymorphism on this methylation in patients with breast cancer. J Fasa Univ Med Sci. 2018; 8 (1) :628-636
URL: http://journal.fums.ac.ir/article-1-1405-en.html


Volume 8, Issue 1 (Spring 2018) Back to browse issues page
Journal of Fasa University of Medical Sciences
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