:: Volume 2, Issue 4 (Winter 2013) ::
J Fasa Univ Med Sci 2013, 2(4): 259-265 Back to browse issues page
Molecular Typing of Acinetobacter Baumannii Clinical Strains in Tehran by Pulsed-Field Gel Electrophoresis
Neda Farahani, Reza Mirnejad 1, Zeinab Ahmadi, Nour Amirmozafari, Faramarz Masjedian
1- , rmirnejad@yahoo.com
Abstract:   (32669 Views)

Background & Objective : Currently, Acinetobacter baumannii is an important nosocomial pathogen insofar as its hospital outbreaks have been described from various geographical areas. Since the discrimination of strains within a species is important for delineating nosocomial outbreaks, this study was conducted with the aim of genotyping the A. baumannii clinical strains in Tehran via the pulsed-field gel electrophoresis (PFGE) method, which is the most accurate method used for the typing of bacterial species.

 

Materials & methods: This study was performed on 70 isolates of acinetobacter baumannii isolated from patients from Baqiyatallah, Rasoole Akram, and Milad hospitals in Tehran. Cultural and biochemical methods were used for the identification of the isolates in species level, and then susceptibility tests were carried out on 50 isolates of A. baumannii using the disk diffusion method. The PFGE method was performed on the isolates by Apa I restriction enzyme. Finally, the results of the PFGE were analyzed.

Result: Acinetobacter baumannii strains isolated from hospitals in Tehran showed seven different genetic patterns, two of which were sporadic . Also, genotypic profiles were different in each hospital, and different patterns of genetic resistance to common antibiotics were observed.

Conclusion: A lthough diversity was observed among the strains of A. baumannii by the PFGE method in Tehran, no epidemic strains were found among them.

 

Keywords: Pulsed-field gel electrophoresis (PFGE), Acinetobacter baumannii, Genotyping
Full-Text [PDF 826 kb]   (4863 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2013/03/13 | Accepted: 2013/09/14 | Published: 2013/09/14


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Volume 2, Issue 4 (Winter 2013) Back to browse issues page