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:: Volume 8, Issue 1 (Spring 2018) ::
J Fasa Univ Med Sci 2018, 8(1): 674-682 Back to browse issues page
The comparison of nanoparticle adjuvant with the montanide adjuvant for hyper-immunization to produce anti-snakebite serum
Pariya Maljaee 1 , Hossein Zolfagharian 2, Mahdi Babaie 3 , Naser Mohammadpour Dounighi 4
1- Department of biology, Faculty of science, Payam noor university, Tehran, Iran
2- Razi vaccine and serum research institute, Agricultural research education and extension organization (AREEO), Tehran, Iran , hosseinzolfagharrian@yahoo.com
3- Young researchers and elites club, Science and research branch, Islamic azad university, Tehran, Iran
4- Razi vaccine and serum research institute, Agricultural research education and extension organization (AREEO), Tehran, Iran
Abstract:   (368 Views)

Background & Objective: Currently selection and use of nanoparticle adjuvant is very important. Due to the lack of effective anti-snakebite serums to treat it and high cost of purchasing external anti-snakebite serums, preparation of suitable internal nanoparticles to produce hyper-immunization plasma has become very important. In order to minimize the disadvantages associated with traditionally used adjuvants in ovines and to produce potent antivenum, a comparison was made between various adjuvants for their immune-potentiation capacity and safety.
Material & methods: The present study was conducted in 15 sheep, divided into three groups and hyper-immunized using crude venom of cobra snake (Naja naja oxiana) along with three adjuvants, Montanide, aluminum phosphate and poly methyl methacrylate through subcutaneous route at intervals of a week. Periodic standard safety assessments were done.
Results: The neutralization activity (LD50) of pooled sera samples by 9th week, obtained with aluminum phosphate, Montanide and poly methyl methacrylate groups were 1.5, 2.2 and 2 LD50/ml respectively. The sheep of poly methyl methacrylate group showed minimum local reactions at injection site, while sheep from other two groups exhibited moderate reactions. However, these were transient and reabsorbed or healed subsequently.
Conclusion: Poly methyl methacrylate nanoparticle adjuvant could be a possible alternative to the emulsion adjuvants for primary phase of immunization in antivenomous preparation considering its acceptable immunopotentiation capacity and safety in donor animals.
 

Keywords: Nanoparticle adjuvant, Naja naja oxiana, Poly methyl methacrylate, aluminum phosphate, Montanide
Full-Text [PDF 1128 kb]   (92 Downloads)    
Type of Study: Research | Subject: Toxicology
Received: 2017/06/7 | Accepted: 2017/10/25 | Published: 2018/06/19
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Maljaee P, Zolfagharian H, Babaie M, Mohammadpour Dounighi N. The comparison of nanoparticle adjuvant with the montanide adjuvant for hyper-immunization to produce anti-snakebite serum. J Fasa Univ Med Sci. 2018; 8 (1) :674-682
URL: http://journal.fums.ac.ir/article-1-1470-en.html


Volume 8, Issue 1 (Spring 2018) Back to browse issues page
Journal of Fasa University of Medical Sciences
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