Volume 10, Issue 1 (4-2020)                   JABS 2020, 10(1): 2081-2093 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khovarnagh N, Seyedalipour B, taravati A. Investigating the Protective Role of N-Acetyl Cysteine and S-Methyl Cysteine in the Activity of Antioxidant Enzymes and the Level of lipid Peroxidation Due to Acetamiprid Administration in Rat Kidney and Serum. JABS 2020; 10 (1) :2081-2093
URL: http://jabs.fums.ac.ir/article-1-1596-en.html
1- Department of Cellular and Molecular Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
2- Department of Cellular and Molecular Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran , b.alipour81@gmail.com
Abstract:   (3179 Views)

Background & Objective: Acetamiprid (ACP), as a neonicotinoid toxin, causes free radicals production and oxidative stress in various organisms. The aim of this study was to evaluate the antioxidative effects of N-acetylcysteine (NAC) and S-methylcysteine (SMC) on reducing acetaminoprid-induced oxidative stress in serum and kidney of rats.
Materials & Methods: In this experimental study, 42 male Wistar rats were randomly divided into 6 groups of 7 including one control, one sham (normal saline) and five experimental groups, which intra-peritoneally received ACP (5 mg/kg), NAG (160 mg/kg), SMC (100 mg/kg), ACP+NAC, ACP+SMC, and ACP+ NAC+SMC for one week. After separation of serum and kidney tissue, the activity of the catalase (CAT), glutathione S-transferase (GST), glutathione concentration (GSH), malondialdehyde (MDA) and total antioxidant (TAC) was determined.
Results: Acetamiprid caused significant increase in GST activity in serum and kidney (p< 0.01), CAT activity in serum and kidney (p< 0.05) but insignificant increase in MDA level and insignificant decrease in GSH and TAC compared to control. NAC and SMC, alone and in combination with ACP, restored the levels of TAC, GSH and MDA and activities of CAT and GST.
Conclusion: Acetamiprid increases lipid peroxidation, activity of CAT and GST, and decreases the concentration of GSH and TAC, presumably by producing free radicals. Administration of NAC and SMS as antioxidants causes a decrease in acetamiprid toxicity due to relative reduction of free radicals.
 

Full-Text [PDF 1433 kb]   (952 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2017/11/22 | Accepted: 2018/10/13 | Published: 2020/02/27

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)