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:: Volume 3, Issue 2 (Summer 2013) ::
J Fasa Univ Med Sci 2013, 3(2): 104-110 Back to browse issues page
Comparison PCR Method and Rapid Urease Test to Detect Helicobacter Pylori in the Gastric Biopsy Tissue Samples
Parastoo Chamanrokh 1, Mohammad hassan Shahhosseiny2, Mahnaz Mazaheri Assadi3, Taher Nejadsattari4, Davood Esmaili4
1- Department of Biology, Science and Research Branch - Islamic Azad University,Tehran, Iran , p.chamanrokh@gmail.com
2- Department of Microbiology, Islamic Azad University, Qods branch, Tehran, Iran
3- Environmental Biotechnology Group, Biotechnology Department, Iranian Research Organization for Science and Technology,Tehran, Iran
4- Department of Biology, Science and Research Branch - Islamic Azad University,Tehran, Iran
Abstract:   (17015 Views)
Background & Objective: Helicobacter pylori has been recognized as a major risk factor in gastric and duodenum ulcers and gastric cancer. Some laboratory tests, such as culture, are not entirely satisfying. The aim of this study is to compare RUT with PCR in identifying H. pylori in the gastric biopsy tissue samples.
Materials & Methods: First, the standard H.pylori sample (N:oC30) was provided. primers or the glmM (ureC) gene were used In PCR method The PCR test was optimized by sensitivity and specificity methods. Then 100 clinical samples composed of stomach tissue biopsy were prepared. The rapid urease test was conducted on all obtained samples to identify H.pylori. DNA was extracted from samples using DNG plus technique. Then, samples were studied using PCR method. 
Results: The 294 bp product was amplified in the optimized PCR test. The PCR test sensitivity was obtained at 10 CFU. Any unwanted products were not seen in the specificity test with the exception of H. pylori DNA samples. Of 100 stomach biopsy samples, 64% were reported as positive by RUT and 76% by the PCR test.
Conclusion: Given that the PCR test has higher sensitivity and specificity to detect H.pylori comparing rapid ureaas test, therefore this method could be used to detect H.pylori.  
Keywords: Helicobacter pylori, glmM, PCR method, rapid Ureas test
Full-Text [PDF 1144 kb]   (6304 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2013/04/10 | Accepted: 2013/10/6 | Published: 2013/10/6
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Chamanrokh P, Shahhosseiny M H, Mazaheri Assadi M, Nejadsattari T, Esmaili D. Comparison PCR Method and Rapid Urease Test to Detect Helicobacter Pylori in the Gastric Biopsy Tissue Samples. J Fasa Univ Med Sci. 2013; 3 (2) :104-110
URL: http://journal.fums.ac.ir/article-1-163-en.html


Volume 3, Issue 2 (Summer 2013) Back to browse issues page
Journal of Fasa University of Medical Sciences
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