:: Volume 9, Issue 1 (Spring 2019) ::
J Fasa Univ Med Sci 2019, 9(1): 1289-1300 Back to browse issues page
The Evaluation of the effects of cytotoxic, anti-proliferative and anti-migrative effects of the thymoquinone on PC3 prostate cancer cell line
Iraj Khodadadi1 , Gholamreza Shafiee 2
1- Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , g.r_shafiee@yahoo.com
Abstract:   (658 Views)
Background & Objective: Herbal medicine and their composition are widely used for different therapeutic purposes. Therefore, this study was designed to evaluate of the anti-proliferation and anti-migration effects of Thymoquinone (TQ), an active part of Nigella sativa, on PC3 prostate cancer cells.
Materials & Methods: In this experimental study, PC3 prostate cancer cells were treated with 0, 10, 30, 50, 70 and 90 µM concentrations of thymoquinone for 12, 24 and 48 hours and cell viability was determined by MTT assay. IC50 concentration of thymoquinone was obtained as 40 µM concentration of thymoquinone and 24h incubation time. PC3 cell migration was studied using the cell migration assay. Colony formation assay was used to determine cell proliferation and Gimsa staining was performed to observe morphological changes of the cells.
Results: The IC50 of Thymoquinone was found in concentration of 40 µM at 24h treatment. Thymoquinone significantly (p<0.05) inhibited cell growth and proliferation as well as cell migration. Treatment of PC3 cells with 40 µM thymoquinone showed significant changes in nucleus and cytoplasm of the cells which are representative of apoptosis and cell death.
Conclusion: Thymoquinone has significant anti-proliferative and anti-migrative effects on prostate PC3 cells and therefore may be used as a complementary agent for prevention of prostate cancer.
 

 
Keywords: Prostatic neoplasms, Thymoquinone, Migration, Colony formation
Full-Text [PDF 1444 kb]   (126 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2018/04/18 | Accepted: 2018/11/7 | Published: 2019/06/8


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Volume 9, Issue 1 (Spring 2019) Back to browse issues page