Journal of Advanced Biomedical Sciences
JABS
Sat, Apr 20, 2024
[Archive]
Volume 9, Issue 4 (11-2019)
JABS 2019, 9(4): 1879-1885 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Tahmasebi Fard Z. The Effect of the Polymorphism on the 5' Region of the Leptin gene (G2548A) on the Possibility of Breast Cancer in two Studies Groups. JABS 2019; 9 (4) :1879-1885
URL: http://jabs.fums.ac.ir/article-1-2006-en.html
URL: http://jabs.fums.ac.ir/article-1-2006-en.html
Departman of Biology, Roudehen Branch, Islamic Azad of university, Roudehen, Iran , ztahmasebi@riau.ac.ir
Abstract: (2646 Views)
Background & Objective: Leptin a hormone secreted from adipose tissue plays a role in regulating energy homeostasis. Polymorphism in the gene structure can alter serum levels of hormone and affect cell function. In this study, the relationship between G2548A polymorphism of the leptin gene with the risk of breast cancer in patients compared with healthy subjects was investigated.
Materials and Methods: The blood sample of 158 women with breast cancer and 158 healthy women of the same age was collected and after extracting DNA and amplifying specimens with specific primers, some part of products was digested with HhaI restriction enzyme to determine the genotypes of individuals based on the number of bands formed on the gel. The results were analyzed by statistical analysis including chi-square, and logistic regression.
Results: The mean age of the patients was 56.00±0.62 years old and the control groups was 55.45±0.72 years old (p-value =0.037). There was a significant relationship between the smoking level of the two groups (p-value =0.026). The history of relatives of breast cancer patients was positive in 43 (27.22%) patients. Analysis of the results of genotypes showed that the mutant homozygote genotype AA had a significant relationship in two groups and increased by 1.686 the risk of breast cancer (P-Value=0.036, OR:1.686, CI95%:1.033-2.753).
Conclusion: The frequency of mutant A allele in the patient group was more than that of the control groups and increased the risk of breast cancer by 1.763 times in carriers. Overall, it can be said that this polymorphism is probably related to breast cancer.
Materials and Methods: The blood sample of 158 women with breast cancer and 158 healthy women of the same age was collected and after extracting DNA and amplifying specimens with specific primers, some part of products was digested with HhaI restriction enzyme to determine the genotypes of individuals based on the number of bands formed on the gel. The results were analyzed by statistical analysis including chi-square, and logistic regression.
Results: The mean age of the patients was 56.00±0.62 years old and the control groups was 55.45±0.72 years old (p-value =0.037). There was a significant relationship between the smoking level of the two groups (p-value =0.026). The history of relatives of breast cancer patients was positive in 43 (27.22%) patients. Analysis of the results of genotypes showed that the mutant homozygote genotype AA had a significant relationship in two groups and increased by 1.686 the risk of breast cancer (P-Value=0.036, OR:1.686, CI95%:1.033-2.753).
Conclusion: The frequency of mutant A allele in the patient group was more than that of the control groups and increased the risk of breast cancer by 1.763 times in carriers. Overall, it can be said that this polymorphism is probably related to breast cancer.
Keywords: Breast Cancer (BC), Leptin, single nucleotide polymorphism, Restriction Fragment
Length Polymorphism (RFLP)
Type of Study: Research |
Subject:
Medical Genetics
Received: 2019/04/20 | Accepted: 2019/06/22 | Published: 2020/03/15
Received: 2019/04/20 | Accepted: 2019/06/22 | Published: 2020/03/15
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)