:: Volume 5, Issue 1 (Spring 2015) ::
J Fasa Univ Med Sci 2015, 5(1): 51-61 Back to browse issues page
Histopathological Evaluation of the Embryo and Weight Assessment of Body, Kidney, and Liver in Pregnant NMRI Mice Exposed to Zinc Oxide (ZnO)Nanoparticles
Bagher Seyedalipour 1, Masumeh Oshrieh2, Ramezan Khanbabaee2
1- Department of Cellular and Molecular Biology, faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. , b.seyedalipour@umz.ac.ir
2- Department of Cellular and Molecular Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
Abstract:   (10887 Views)


Background & Objective: Zinc is an essential trace element which plays a key role in the growth and the development of the embryo during pregnancy. This study was designed to investigate the cytotoxic effects of zinc oxide nanoparticles on embryonic development and to assess the weight of body, kidney, and liver in Naval Medical Research Institute (NMRI) mice.

  Materials & Methods: 25 female of NMRI mice weighting 30±3.0 gram were randomly divided into five groups (five in each group, four experimental groups and one control group). Mice in experimental groups one, two, three, and four received intraperitoneal ZnO nanoparticle with the concentrations of 50,100,150, and 200 mg / kg, respectively during 15 days (every other day). At the end, the weight of the body, the kidney, and the liver of the pregnant mice and the embryos were measured. In addition, histopathological evaluations were performed on embryos. The data were analyzed by SAS software in P≤0.05.  

Results: Based on the macroscopic observations, the embryo and the kidney weights decreased and increased, respectively with increasing different concentrations of nanoparticle compared with controls (P≤0.05). Our data showed that at different concentrations of nanoparticles, the distance, the size, and the number of vertebral bodies increased compared to the control group. At the concentration of 150 mg/kg, an accumulation of mesenchymal cells for cartilage were observed and it seems that high dose of nanoparticles prevents embryo growth.

Conclusion: The results of this study indicate that ZnO nanoparticles cause embryonic developmental delay, undifferentiated and disorganized vertebral bodies in NMRI mice.


Keywords: zinc oxide nanoparticles, NMRI mice, embryonic development
Full-Text [PDF 1036 kb]   (2268 Downloads)    
Type of Study: Research | Subject: Embryology
Received: 2014/10/20 | Accepted: 2015/01/30 | Published: 2015/06/14

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Volume 5, Issue 1 (Spring 2015) Back to browse issues page