:: Volume 5, Issue 2 (Summer 2015) ::
J Fasa Univ Med Sci 2015, 5(2): 219-228 Back to browse issues page
The effects of Intracerebellumal Injection of Estrogen on Anxiety-like Behavior and Parameters of Antioxidant System following Ethidium Bromide Injection in rat
Faranak Nejati1, Shiva Khezri 2, Homeira Hatami3, Ali reza Ali hemmati4
1- Department of Biology, Facultyof Science, Urmia University, Urmia, Iran.
2- Department of Biology, Facultyof Science, Urmia University, Urmia, Iran. , skhezri72@gmail.com
3- Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
4- Department of Anatomy and Histology, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran.
Abstract:   (6362 Views)

Background & Objectives: Demyelination by Ethidium bromide is one of the ways that is usually used for preparing experimental models of MS. Treatment with estrogen protects oligodendrocyts from cell poisoning and stops lipidic peroxidation. The aim of this study is the evaluation of the effects of estrogen on anxiety like behavior and stress oxidative parameters after inducing demyelination by ethidium bromide to the cortex of cerebellum in rats.


Materials & Methods: Induction of demyelination was carried out in cerebellum cortex by direct injection of Ethidium Bromide (EB) (0.01%). One week after injection of EB, animals were treated with two doses of estrogen (2µg/μl and 4µg/μl) for 3 days. After the treatment stage, characteristics of anxiety were assessed by using Elevated plus Maze  moreover , the parameters of stress oxidative were evaluated. One-way Analysis of Variance (ANOVA) was performed.


Results: The results of this study showed that Open Arm Entry percentage and open arm time (anxiety index) in rats with estrogen treatment was significant relative to Ethidium Bromide group (p<0.01) in addition , activity value of glutathione peroxidase and catalase showed significant increase compared to Ethidium Bromide group.

Conclusion: Microinjection of estrogen can decrease anxiety as well as oxidative stress.

Keywords: Ethidium Bromide, Estrogen, Anxiety, Cerebellum, Oxidative stress, Rat
Full-Text [PDF 1070 kb]   (1793 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2014/11/16 | Accepted: 2015/03/3 | Published: 2015/09/13

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Volume 5, Issue 2 (Summer 2015) Back to browse issues page