Volume 5, Issue 3 (11-2015)                   JABS 2015, 5(3): 387-396 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Evaluation of inhibitory effects of Chlorella vulgaris extract on growth, proliferation and biofilm formation by Streptococcus mutans and evaluation of its toxicity . JABS 2015; 5 (3) :387-396
URL: http://jabs.fums.ac.ir/article-1-815-en.html
Abstract:   (9988 Views)

Background & Objectives: Dental caries is the most important disease caused by some bacteria specially Streptococcus Mutans from Viridans family. The aim of this study is to evaluate the inhibitory effect of Chlorella vulgaris extract on growth, proliferation, and biofilm formation of Streptococcus mutans.

Materials & Methods: Microalgae Chlorella vulgaris was extracted via maceration using chloroform, methanol, and acetone (2/1/1) as solvents. The antibacterial activities were evaluated through methods such as disk diffusion, well diffusion, minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) besides, the anti-biofilm formation of Chlorella vulgaris extract was indicated. In the present study, a method utilizing brine shrimp lethality was used to screen the toxicity of Chlorella vulgaris extract, and the mortality of brine shrimps was counted by Magnifying glass in a 24-hour period.

Results: The results of disc diffusion and well diffusion of Chlorella vulgaris extract revealed the averages of 16.5 and 23 mm zones of inhibition in Mueller-Hinton agar, respectively. The minimal inhibitory concentration was 25 mg/ml and the minimal bactericidal concentration was 50 mg/ml. The anti-biofilm formation concentration of Chlorella vulgaris extract was 50 mg/ml, and the concentration of brine shrimp toxicity was100 mg/ml.

Conclusion: The present study showed that Chlorella vulgaris extract has more significant antimicrobial properties than ampicillin and is able to eliminate Streptococcus mutans biofilm.

Full-Text [PDF 958 kb]   (5101 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2015/05/18 | Accepted: 2015/07/7 | Published: 2015/11/30

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)