TY - JOUR T1 -  Effects of Aerobic Interval Training and Adenosine on the Expression of AMPK, PPARγ and A2B Receptor in the Liver of Rats Fed a High-Fat Diet TT - اثر تمرین تناوبی هوازی و آدنوزین بر بیان AMPK، PPARγ و گیرنده A2B کبد در موش‌های صحرایی تغذیه‌شده با غذای پرچرب JF - JABS JO - JABS VL - 10 IS - 3 UR - http://jabs.fums.ac.ir/article-1-2239-en.html Y1 - 2020 SP - 2655 EP - 2664 KW - aerobic interval training KW - adenosine KW - high-fat diet KW - AMPK KW - PPARγ KW - A2B KW - rat N2 - Background & Objective: Obesity causes steatosis in the liver. PPARγ transcription factor of glucose and fat metabolism genes and AMPK cell energy sensor are considered as therapeutic targets in non-alcoholic fatty liver (NAFLD) patients. Despite the importance of exercise and medication in the treatment of chronic diseases, there is little agreement on the role of intense exercise (HIIT) in the treatment of NAFLD. The aim of this study was to investigate the effect of exercise intensity and adenosine on liver fat metabolism following a high-fat diet. Materials & Methods: 30 mice were randomly divided into 6 groups of 5 controls, high fat diet, high fat diet + placebo injection, high fat diet + adenosine injection, high fat diet - exercise + adenosine injection, high fat diet - exercise + placebo injection. Subjects first received 13 weeks of high-fat diet and then exercised 5 sessions per week for 12 weeks. Result: PPARγ gene expression increased in high-fat food groups (P = 0.017), (P = 0.007). There was no difference in AMPK expression between food groups (P = 0.097). Exercise had a significant effect on increasing expression of AMPK, A2B (P = 0.0001), (P = 0.031) and a significant decrease on PPARγ (P = 0.0001). In adenosine group, AMPK and A2b significantly increased (P = 0.001), (P = 0.012) and PPARγ decreased (P = 0.0001). Simultaneous exercise and adenosine had a more significant increase in AMPK expression (P = 0.039) and a more significant decrease in PPARγ (P = 0.005) than the effect of each alone. Conclusion: It is possible to use high-intensity exercise with a certain amount of adenosine at the same time to prevent the accumulation of fat in the liver. Keywords: M3 ER -