TY - JOUR JF - JABS JO - JABS VL - 5 IS - 1 PY - 2015 Y1 - 2015/4/01 TI - Evaluation of Antibacterial Effect of Curcumin Loaded Chitosan Nanoparticles TT - ارزیابی اثر ضد باکتریایی نانو ذرات کایتوزان بارگذاری شده با کورکومین N2 - Background & Objective: Nanomedicine delivery systems are known as potent therapeutictools. In addition to possessing an effective agent of turmeric, Curcumin shows antibacterial properties as well. Curcumin is not water-soluble and it can be solved in water via nanotechnology-base methods. Chitosan is a natural and biodegradable substance that is utilized for the Production of Nanoparticles (NPs) carrying drug. In the following investigation, curcumin is loaded in chitosan NPs and ultimately, the resulting nano-drug is studied as an antibacterial agent. <span style="line-height: 115% font-family: " b="" nazanin"="" font-size:="" 10pt="" mso-ascii-font-family:="" "times="" new="" roman"="" mso-hansi-font-family:="" roman""="" dir="rtl"> Materials & Methods: In this study, NPs are produced using chitosan and Tripolyphosphate (TPP) salt. Curcumin solution was loaded in chitosan NPs during their production. Next, the skins of BALB/c mice infected with staphylococcus aureus are treated by curcumin-loaded chitosan NPs for 3 days. Afterwards, in order to evaluate the antibacterial property of the nano-drug, these skin suspensions of mice are cultured in bacterial medium. Results: Dynamic Light Scattering (DLS) reveals the charge of + 7 ± 2 mV and the size of 160 ± 10 nm in curcumin-loaded chitosan NPs. Moreover, Transmission electron microscopy (TEM) and atomic force microscopy (AFM) indicates a spiral shape. Therefore, the evaluation of the optical density by spectrophotometry demonstrates that 75 ± 2 % of curcumin are loaded in chitosan NPs. Bacterial culture shows that curcumin-loaded chitosan NPs significantly inhibited staphylococcus aureus growth. Conclusion: This study demonstrates that curcumin-loaded chitosan NPs can be applied as a potent agent in treatment of bacterial skin infections. SP - 134 EP - 141 AU - Mofazzal Jahromi, Mirza Ali AU - Rajayi, Hajar AU - Al-Musawi, Sharafaldin AU - Pirestani, Majid AU - Fasihi Ramandi, Mahdi AU - Ahmadi, Kazem AU - Sharifzadeh Peivasti, Vahid AU - Mohammad Hassan, Zuhair AU - Kamali, Mahdi AU - Mirnejad, Reza AD - Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. KW - Nanoparticles KW - Curcumin KW - Chitosan KW - staphylococcus aureus UR - http://jabs.fums.ac.ir/article-1-466-en.html ER -