Journal of Advanced Biomedical Sciences
مجله علوم زیست پزشکی پیشرفته
JABS
Medical Sciences
http://jabs.fums.ac.ir
1
admin
2228-5105
2783-1523
8
7
14
8888
13
en
jalali
1399
8
1
gregorian
2020
11
1
10
4
online
1
fulltext
fa
بررسی فراوانی ژنهای متالوبتالاکتاماز blaVIM1 و blaIMP1 در سویههای سودوموناس آئروژینوزای جداشده از بیماران بستری در بیمارستانهای شهر ارومیه به روش ژنوتیپی
Prevalence of the Metallo-β-Lactamase, BlaIMP1 and BlaVIM1 Genes in Pseudomonas Aeruginosa Isolated from Hospitalized Patients in Urmia Hospitals
ميكروبیولوژی
Microbiology
پژوهشي
Research
<div style="text-align: justify;"><strong><em>Background & Objective:</em></strong> <em>Pseudomonas aeruginosa</em> is one of the gram-negative, opportunistic, catalase-positive pathogens that causes important opportunistic infections such as urinary tract infection, pneumonia, and septicemia. It is resistant to many antibiotics, including third-generation cephalosporins, penicillin and cephamycin. The prevalence of the Metallo-β-lactamase, bla<sub>IMP1</sub> and bla<sub>VIM1</sub> genes in <em>Pseudomonas Aeruginosa</em> isolated from hospitalized patients in Urmia hospitals has not been investigated yet. The aim of this study was to determine the prevalence of bla<sub>VIM1</sub> and bla<sub>IMP1</sub> metallo-β-lactamase genes in clinical isolates of <em>Pseudomonas aeruginosa f</em>rom hospitalized patients in Urmia<span dir="RTL">.</span><br>
<strong><em>Materials & Methods:</em></strong> In this descriptive cross-sectional study, from May to August 2019, a total of 75 P. aeruginosa from different sections (urine, wound and lung secretions) were collected from Urmia medical centers. After bacterial confirmation by biochemical tests, antibiotic susceptibility tests were performed using the disc release method. Identification of blaVIM1 and blaIMP1 genes in the samples was performed using PCR method.<br>
<strong><em>Results:</em></strong> The results of the antibiotic susceptibility test showed 86.6% of resistance to beta-lactam drugs in the isolates. In this study, the highest resistance to cefotaxime (86.6%) and the lowest resistance to gentamicin was observed (16%). Based on the PCR results, 55.38% of isolates contained bla<sub>IMP1</sub> gene and 0% contained bla<sub>VIM1</sub><span dir="RTL">.</span><br>
<strong><em>Conclusion:</em></strong> The result of this study showed a large percentage of <em>Pseudomonas aeruginosa</em> strains were resistant to carbapenem antibiotics, and bla<sub>IMP1</sub> was dominant gene among carbapenem-resistant strains. Since the importance of Metallo-β-lactamase generating strains in the hospitals, rapid identification of these strains can be an important step in the treatment and control of infections caused by these strains.<br>
</div>
Pseudomonas aeruginosa, Metallo-β-lactamase, Hospital infections, Antibiotic resistance, PCR
2682
2692
http://jabs.fums.ac.ir/browse.php?a_code=A-10-2655-1&slc_lang=fa&sid=1
Mahsa
Abedi
مهسا
عابدی
mahsa.abedi274@yahoo.com
3740511051
100319475328460023877
No
Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran
گروه زیستشناسی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران
Maryam
Parvini Kohnehshahri
مریم
پروینی کهنه شهری
parvini29@gmail.com
2851707558
100319475328460023878
Yes
Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran
گروه زیستشناسی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران