Journal of Advanced Biomedical Sciences
مجله علوم زیست پزشکی پیشرفته
JABS
Medical Sciences
http://jabs.fums.ac.ir
1
admin
2228-5105
2783-1523
8
7
14
8888
13
en
jalali
1400
6
1
gregorian
2021
9
1
11
3
online
1
fulltext
en
ارتباط بین تشکیل بیوفیلم با حضور ژن های متالوبتالاکتامازی و کارباپنمازی در جدایه های بالینی کلبسیلا پنومونیه بیمارستان های شهر قم
Relationship between Biofilm and the Presence of Drug Resistance Genes in Clinical Isolates of Klebsiella Pneumoniae in Qom Hospitals
ميكروبیولوژی
Microbiology
پژوهشي
Research
<div style="text-align: justify;"><strong><em>Background & Objectives:</em></strong> <em>Klebsiella pneumoniae</em> is a significant cause of nosocomial infections. This bacterium survives in difficult conditions by forming biofilms in hospital equipment and causes severe infections. On the other hand, the emergence and spread of carbapenem resistance among bacteria and biofilm production is a current health concern. There are controversial findings about the relevance of this issue. This study aimed to evaluate the relationship between biofilm formation and carbapenem resistance among clinical isolates<strong>.<span dir="RTL"></span></strong><br>
<strong><em>Materials & Methods</em></strong><strong>:</strong> A total of 160 isolates of <em>Klebsiella pneumoniae</em> were collected. Molecular methods were used to detect resistance genes. Subsequently, the ability to produce biofilms in isolates with resistance genes was assessed. Finally, the correlation of biofilm formation among resistant isolates was calculated using χ2 test<strong><span dir="RTL">.</span></strong><br>
<strong><em>Results:</em></strong> 79 imipenem-resistant isolates were obtained. 46 isolates (66.66%) containing VIM gene, 36 isolates (52.17%) containing OXA-48 gene, five isolates (7.24%) containing NDM gene, Six isolates (8.69%) containing gene IMP and five isolates (7.24%) also had KPC gene. The results showed a significant correlation between the ability to form biofilms and the presence of carbapenem-resistant genes<strong>.</strong><br>
<strong><em>Conclusion:</em></strong> Increased carbapenem resistance in <em>Klebsiella pneumoniae</em> isolates and its association with biofilm formation is severe warning for basic measures to combat this phenomenon.<span dir="RTL"> </span><br>
</div>
Klebsiella pneumoniae, biofilm, drug resistance, carbapenemase gene, metallobetalactamase gene
3951
3957
http://jabs.fums.ac.ir/browse.php?a_code=A-10-2919-2&slc_lang=en&sid=1
Seyyed Soheil
Aghaei
سید سهیل
آقایی
soheilaghaee@yahoo.com
0056868381
100319475328460026402
Yes
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران
Ali
Javadi
علی
جوادی
0381418138
100319475328460026403
No
Department of Medical Science, Qom Branch, Islamic Azad University, Qom, Iran
گروه علوم پزشکی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران