@article{ author = {Nazari, Razieh and Sattarahmady, Naghmeh and Heli, Hossei}, title = {Nano-technological methods for increasing the oral bioavailability of curcumin}, abstract ={Background & Objective: Curcumin is a tropical plant in the ginger (Zingiberaceae) family. Three curcuminoids were from turmeric: Curcumin, demethoxycurcumin, and Bisdemethoxycurcumin. The potential health benefits of curcumin are limited by their poor solubility, hydrophobic property, and low absorption from the gut, rapid metabolism and rapid systemic elimination. Materials & Methods: Scholarly articles were selected using valid keywords and searching on the SID, Google Scholar, PubMed and Elsevier databases. The aim of this paper is to introduce Nano-technological techniques for increasing the oral bioavailability of curcumin. Results: Increase in the drug bioavailability is one of the goals of nanotechnology. Nano-scale materials increase the drug bioavailability due to their unique features. The bioavailability of a drug is defined as a percentage of the initial dose that is independent of the administration route of the drug entering the bloodstream. To improve the bioavailability of curcumin, numerous techniques are used such as curcumin liposome complex, curcumin phospholipid nanoparticles and structural analogues of curcumin. Conclusion: Curcumin has a unique biological and pharmaceutical property. Of course, one of the major limitations of curcumin is its instability and its low solubility. Thus, it is useful to provide a method that can increase the solubility of poorly soluble drugs in water and protect them to achieve the target site. Nanotechnology has provided many ways to improve the solubility and transport of these drugs.  }, Keywords = {Curcumin, Biological Availability, drug delivery, Nanotechnology}, volume = {7}, Number = {2}, pages = {152-161}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1288-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1288-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Tavakoli, Ahmad and KarbalaieNiya, Mohammad Hadi and Keshavarz, Mohsen and Ghaffari, Hadi and Esghaei, Maryam}, title = {Mechanisms Involved in Immunopathogenesis of influenza virus infection}, abstract ={Influenza epidemics and pandemics cause notable morbidity and mortality.  Mortality cases are mostly associated with immunopathogenic mechanisms, although still poorly understood. Human studies help to understand the immunopathogenesis of influenza. However, there is limited information in this regard. Recent studies using experimental animal models have significantly improved our knowledge on complex mechanisms involved in the immunopathogenesis during influenza infections including acute inflammatory responses of neutrophils, dendritic cells, macrophages, toll-like receptors, chemokines, cytokines, CD4+ and CD8+ T cells. Due to influenza infection, elevated levels of cytokines and chemokines are produced during influenza-induced inflammation which are known as cytokine storm which is a severe immune response characterized by the recruitment of inflammatory leukocytes and increased levels of cytokines and chemokines at the site of infection. This review aimed to discuss the most recent findings on mechanisms of influenza immunopathogenesis.  }, Keywords = { Influenza, Immunopathogenesis, Innate immunity, Adaptive immunity, Antiviral immune response}, volume = {7}, Number = {2}, pages = {162-171}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1152-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1152-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Sheikhanishahin, Homa and Mehrabani, Golnoosh and Rezaei, Rasoul and Karimi, Mohsen and Amini, Masou}, title = {The Effect of Aerobic Exercise on Renal Function and Metabolic Syndrome in Kidney Transplant Athletes}, abstract ={Background & Objectives: The relationship between metabolic disorders and renal functions in kidney transplant recipients has been reported. Considering the identification of many advantages of regular physical activities in maintaining health, for chronic kidney patients, the researchers tried to study the effect of aerobic activities on renal function and metabolic syndrome in kidney transplant athletes. Materials & Methods: Twenty female kidney transplant athletes were randomly selected and divided into two equal groups of experimental (mean of age =24.5±2.7 years, height=161±4.2 cm and weight= 57.9±1.8 kg) and control (mean of age =24.9±2.3 years, height=162±2.4 cm and weight= 59.5±4.02 kg). The experimental group carried out an exercise for eight weeks (three sessions per week). At the end of eighth week, the renal function was assessed based on glomerular filtration rate and metabolic syndrome indices. Paired-samples t-test was used to compare the data before and after physical exercise and independent-samples t-test was used to compare the two groups with the significance level of p< 0.05. The eight weeks of aerobic exercise did not have any significant impact on lipid profile levels, while it caused a significant decrease in glomerular filtration rate and fasting blood sugar in kidney transplant athletes in the experimental group after exercise. Conclusion: The results indicated that physical exercise can be considered as a good way of changing glomerular filtration rate and controling fasting blood sugar in athletes undergoing kidney transplant}, Keywords = {Renal Function, Metabolic Syndrome, Kidney Transplant, Athletes}, volume = {7}, Number = {2}, pages = {172-180}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1133-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1133-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {HassanvandAmouzadeh, Mahdi}, title = {Evaluation of validity and reliability of social phobia inventory among students in Payame Noor University of Lorestan}, abstract ={Background & Objective: Social phobia has been a prevalent psychiatric disorder which is associated with a wide range of negative effects of different life aspects of affected people. Developing a simple tool is necessary to assess the social phobia. The aim of the study was to assess the reliability and validity of the social phobia inventory (SPIN) among students in Payame Noor University of Lorestan. Material & Methods: In a correlational study, 410 students of Lorestan Payame Noor University were selected by clustering sampling method in the first semester of academic year 2015–16. The evaluation of reliability was conducted using Cronbach's alpha, split-half reliability, Spearman-Brown coefficient and test - retest coefficient and in order to assess the validity of this instrument, the relation of subscales of SPIN with other four scales including anxiety from the symptom checklist-90-revised (scl-90-R), cognition error questionnaire (CEQ), self-esteem rating scale (SERS), and multidimensional body-self relations questionnaire (MBRSQ) was investigated using Pearson correlation coefficient method. Results: Convergent validity of the SPIN with anxiety subscales of the (SCL- 90-R) and (CEQ) were respectively r= 0.83, r=0.47and discriminant validity of the SPIN with (SERS) and (MBRSQ) were respectively r= -0.70, r=-0.44 (α<0.001). The reliability of the SPIN with Cronbach’s alpha, split-half reliability, Spearman-Brown coefficient and test-retest coefficient for the total scale  were 0.98, 0.94, 0.96, 0.84 respectively (α<0.0001). Conclusion: It seems that the SPIN can be used as a valid and reliable tool for researchers and specialists in the field of mental health.  }, Keywords = {Social Phobia Inventory, Validity, Reliability, Anxiety, Students}, volume = {7}, Number = {2}, pages = {181-189}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1026-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1026-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Mobasheri, Farzaneh and Jafarzadeh, Saeedeh and Bahramali, Ehs}, title = {The Level of Satisfaction of Family Physician Program in Urban Population of Fasa from 2014 to 2015}, abstract ={Background & Objective: Patient satisfaction is an indicator of the health services that is increasingly important in today's competitive world and perhaps the simplest method for evaluation of family physician program. This study aimed to determine the level of satisfaction of service recipients in urban areas in Fasa, Iran, with implementation of the Family Physician Program. Materials & Methods: This descriptive cross-sectional study was carried on the 411 patients for whom family physician program was implemented in 2014. Data were collected by a valid and reliable demographic and satisfaction questionnaire in 6 dimensions (care and reverence, trust and belief in his performance, waiting time, availability and timely access to a doctor in time, usefulness of actions, payment costs and paramedical unit performance and on time accessibility, professional services and emergency). Data were analyzed using SPSS software version 18 using independent-samples t-test, chi square test, and ANOVA. Results: Only 1.7% of people had little satisfaction. However, the majority of people expressed consent in all aspects of the Satisfaction of the Family Physician Project. The highest levels of satisfaction were observed in care, respect, trust and belief and the emergency services were observed in the lowest level of satisfaction. Conclusion: Good satisfaction after implementing the family physician program shows the successfulness of the program. Therefore, implementation of family physician program will be a significant milestone for other cities but in order to increase the satisfaction of the emergency services and relevant programs, revision seems necessary in order to improve some of the indices of people satisfaction.      }, Keywords = {Satisfaction, Service recipients, Urban Family Physician, Fasa}, volume = {7}, Number = {2}, pages = {190-198}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-988-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-988-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Mohammadpour, Hossein and Razban, Vahid and Mahmoodi, Mahdi and Hajizadeh, Mohammad Reza and Hosseinipour, Maryam and Ghoreishi, Atena Sadat and Farsi, Gity and Hosseini, Farzaneh Sadat and Khoshdel, Ali Rez}, title = {Comparing the effects of fetal bovine and human sera on isolation, expansion and osteogenic differentiation of adipose-derived stem cells}, abstract ={Background & Objectives: Adipose-derived stem cells (ADSCs) have been investigated as a promising cell source for therapeutic and engineering applications. Common proliferation protocols use fetal bovine serum (FBS) as a growth factor which is potentially a source of pathogens, and contains animal antigens that can cause an allergic reaction after transplantation in the recipient, and regarding immunity, human serum (HS) could be a suitable alternative to it. The aim of this study is to compare the culture medium enriched with FBS or HS as supplements in the proliferation and differentiation of ADSCs. Materials & Methods: Human serum was extracted from 90 ml venous blood of a healthy person, taken in respective intervals. The ADSCs were isolated according to the protocol from adipose tissue after lipolysis operation and cultured by mediums enriched with FBS or HS. Expression of the surface markers of ADSCs was investigated by flow cytometry. Osteogenic differentiation was evaluated by Alizarin Red staining method. Results: The analysis of cell growth showed that the isolation and proliferation of ADSCs in both media (HS & FBS) were similar, but there was a significant difference in case of differentiation in the HS medium. Conclusion: This study showed that FBS could be replaced by HS in case of isolation, proliferation and differentiation of stem cells studies as supplement. Furthermore, our data suggest a fast and safe proliferation protocol by using human serum in the stem cells culture and cell-based therapies.  }, Keywords = {adipose-derived stem cells (ADSCs), human serum (HS), fetal bovine serum (FBS)}, volume = {7}, Number = {2}, pages = {199-209}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1024-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1024-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Rashidi, Atousa and Moradli, Gholamali}, title = {Molecular analysis of various classes of integrons in the clinical Pseudomonas aeruginosa isolates by Multiplex-PCR}, abstract ={Background & Objective: The presence of classes I, II and III Integrons in multidrug-resistant Pseudomonas aeruginosa strains, has created new concerns in clinics. This study aimed to investigate the prevalence of class I, II and III Integron in clinical Pseudomonas aeruginosa isolates. Materials & Methods: In the cross-sectional study, 60 isolates of P. aeruginosa were obtained from the microbial-Laboratory of Rasool Akram Hospital in Tehran. Antibiotic susceptibility test was performed by the agar diffusion method based on CLSI guidelines on the Mueller Hinton agar medium. The genomic DNA was extracted using DNA extraction kit and M-PCR was performed for amplification of intI, intII and intIII genes. Results: The highest resistance rate was in the strains that harbored integron, so that 96.6% of strains were resistant to amoxicillin. The results showed that all 60 strains had resistance (100%) to amikacin. The highest frequency was related to intI (40%) and the lowest frequency was related to intIII genes (16.6%). Conclusion: Considering the high prevalence of class I Integron in all Pseudomonas aeruginosa-resistant isolates and its relation with different drug resistance patterns, the right solution to infection control and treatment in hospitals is essential to prevent further spread of it.  }, Keywords = {Class I, II, III Integron genes, Pseudomonas aeruginosa, Multiplex-PCR.}, volume = {7}, Number = {2}, pages = {210-216}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1046-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1046-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Kohan, Leil}, title = {Investigating the association of rs1862513 genetic variant in resistin gene with susceptibility to breast cancer}, abstract ={Background & objective: Resistin is a unique hormone secreted from adipocytes. Recent data indicated that resistin levels have been associated with inflammatory markers; Due to the fact that chronic inflammation plays a role in breast cancer, it seems that resistin may present a molecular link between inflammation and breast carcinogenesis. The aim of this study was to investigate the association of rs1862513 promoter resistin gene variant with breast cancer risk. Material & Methods: For the purpose of this case-control study, 150 breast cancer and 150 healthy control blood samples were collected. After DNA extraction, genotype determination was performed by PCR-RFLP method. Statistical analysis was done using logistic regression model. Results: The case and control groups were in Hardy-Weinberg equilibrium. The subjects with GG genotype had increased risk of breast cancer compared to those with CC genotype (OR: 1.9, 95% CI: 1-3.7, P: 0.04). Also, the G allele indicated a positive association with breast cancer susceptibility (OR: 1.4, 95% CI: 1-1.98, P: 0.03). Conclusion: Results of this study showed possible association of resistin rs1862513 gene variant with breast cancer risk in Iranian women.}, Keywords = {Resistin, Breast cancer, Promoter, Genetic variant}, volume = {7}, Number = {2}, pages = {217-222}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1166-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1166-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Heidari, Ehsan and Peymai, Maryam and Kazemi, Ali and Ghaedi, Kamr}, title = {Codon Optimization of Stem Cell Factor (SCF) gene and evaluation of this gene expression using vector pBudCE4.1 in Chinese Hamster Ovary cells (CHO)}, abstract ={Background & Objective: Stem cell factor (SCF) is a blood cytokine which can play a significant role in the differentiation of blood precursor cells, survival, proliferation and differentiation of mast cells, and it can also increase the proliferation and invasion of tumor cells through affecting hematopoietic cells. Its therapeutic effect in the treatment of diseases such as Alzheimer's and myocardial infarction is being investigated. The aim of this study was to clone SCF gene into the pBudCE4.1 expression vector by codon optimization in Chinese hamster ovary cells (CHO). Materials & Methods: In this experimental study, SCF sequence was made synthetically after codon optimization for CHO cells and changing of the contents of G+C and subcloned into the expression vector pBudCE4.1. Then, SCF gene was transfected into CHO-K1 cells and evaluated using RT-qPCR for gene expression of the relevant gene. Results: Recombinant expression vector pBudCE4.1/SCF was constructed and approved by PCR technique, enzyme digestion and sequencing. RT and PCR results showed that SCF genes can be expressed in CHO cells after codon optimization. Conclusion: The results showed that the cloning of SCF gene can be done well through expression vector pBudCE4.1 and this expression vector can be an appropriate vector for expressing SCF gene into CHO cells.  }, Keywords = {Codon optimization, Cloning, Chinese Hamster Ovary Cells (CHO), Stem Cell Factor (SCF) }, volume = {7}, Number = {2}, pages = {223-232}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1195-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1195-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Fattahi, Esmaeil}, title = {Investigating the effect of Diazinon poison on Sex hormone serum levels and Ovarian Follicle in Female Rats}, abstract ={Background & objective: Diazinon (DZN) as an environmental pollutant adversely effects reproductive system. This study was done to investigate the effect of DZN on sex hormone serum levels and ovarian histological changes in rats. Material & Methods: In this study, 40 female Wistar rats were randomly divided into four groups including experimental groups 1 and 2 that received 5 and 10 mg/kg diazinon intraperitoneally for 4 weeks respectively. The sham group received olive oil, and the control group received no injection. The animals were anesthetized while proestrus phase and blood samples were collected from the heart and the blood serum sex hormones were measured. by Radioimmunoassay (RIA) method. In addition, ovaries were removed and sections were prepared for histological studies. Data were analyzed using one-way ANOVA and Duncan test. Results: The results showed that the levels of estrogen and progesterone, number of corpus luteum, primordial, primary, growing, Graafian follicles and diameter of oocyte, corpus luteum, theca and granulosa thickness significantly decreased in the experimental groups compared to control group (p<0.001). Also, the number of atretic follicles in the experimental groups significantly increased com­pared to that of the control (p<0.001). Conclusion: The results indicated that diazinon causes changes in ovarian tissue and reduces the estrogen and progesterone hormones and can have adverse effects on oogenesis.    }, Keywords = {Diazinon, Ovary, Oocyte, Graafian follicle, Female Rat}, volume = {7}, Number = {2}, pages = {233-240}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1158-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1158-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Afzalahangran, Nahideh and Nekoei, Zahra and Deliraj, Nouroz and AbtahiForoushani, Seyyed Meisam}, title = {The effects of mesenchymal stem cells treated with estrogen on neutrophil cells functions}, abstract ={Background & Objectives: The regulatory role of the mesenchymal stem cells on the cells of immune system such as neutrophil has been investigated in several studies. On the other hand, estrogen hormone is one kind of immunomodulatory agents. This study was designed to investigate the effect of mesenchymal cells treated with estrogen on the neutrophil function. Materials & Methods: Having isolated the mesenchymal stem cells from bone marrow of rats, the researchers treated the cells with 10 or 20 nM concentrations of estrogen for 72 hours. In the next stage, the MSC cells were co-cultured with neutrophil cells and incubated for 1 hour. Then, the neutrophil functions were assessed (P<0/05). Results: The results of the study clearly showed that the percentage of phagocytosis and the respiratory burst in co-cultured neutrophil with mesenchymal stem cells treated with estrogen had increased compared to that of the control group. Moreover, the rate of apoptosis in neutrophils treated with estrogen significantly decreased in both estrogen concentrations compared to that of the control group (P<0/05). Conclusion: It seems that estrogen can modify the interaction of the mesenchymal stem cells and neutrophil.    }, Keywords = {Mesenchymal stem cells, Estrogen, Neutrophil}, volume = {7}, Number = {2}, pages = {241-247}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1028-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1028-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Rabiee, Maryam and Sazgar, Hossein and MohammadiFarsani, Farzaneh}, title = {Bioinformatics analysis of structure and function of Lebestatine isolated from Macrovipera venom}, abstract ={Background & Objective: Lebestatin is a new member of disintegrin family. This single-chain poly peptide comprises 107 amino acids isolated from Macrovipera venom. Lebestatin is connected to integrin, counteracts the adhesion and emigration of endothelial cells through inhibiting the action of this protein and has anti-vessel formation effect. The goal of this study was to survey the interaction of this peptide with integrin and identify important amino acids involved in this interaction. Material & methods: Given the importance of Lebestatin in inhibiting the adhesion and migration of cancer cells, in this study the structure of this peptide was constructed through Phyre2 server and it was compared with other similar peptides. In the next step, in order to identify the mechanisms of the action of this peptide, its interaction with integrin α1 subunit was investigated with Z-Dock server. The results of this interaction were analyzed with Chimera 1.5.3 and SPDBV. Results: The results obtained from simulation showed that the structure of Lebestatin is closely similar to its co-family proteins. Moreover, bioinformatics studies showed that Lys50, Cys71 and Thy92 residues of Lebestatin and Asp20, Tyr17, His118 of integrin α1 subunit are significant amino acids involved in the interaction between Lebestatin and its target integrin. Conclusion: Bioinformatics studies applied in this investigation resulted in identifying significant amino acids involved in the interaction between Lebestatin and its target integrin. Results of this study could facilitate the development of more effective peptides for the inhibition of integrin, metastasis and angiogenesis of cancer cells.  }, Keywords = {Lebestatine peptide, KTS motif, disintegrin, integrin α1 β1}, volume = {7}, Number = {2}, pages = {248-256}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1084-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1084-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Abadi, Neda and Bashiri, Jabbar}, title = {The effect of three-month aerobic training on the expression of AIF and caspase-9 gene in male rat soleus muscle}, abstract ={Background & Objectives: Apoptosis in skeletal muscle plays an important role in disease-related tissue dysfunction such as muscle atrophy. Current evidence suggests that exercise training may alter apoptosis-related signaling and enzymes in skeletal muscle. Therefore, the purpose of this study was to investigate the effect of three-month aerobic training on AIF and caspase-9 gene expression in male rat soleus muscle. Materials & Methods: This study was conducted as a two-group experimental design and sixteen 3-month-old male rats were selected and randomly divided into two groups of aerobic training (n=8) and control (n=8). Rats in trained group participated in the aerobic training program for three months (75-80%). 48 hours after the last training session, the soleus muscle of rats were extracted and AIF and caspase-9 mRNA were evaluated by Real Time-PCR. Independent-samples t-test was applied to analyze the data (P<0.05). Results: AIF gene expression of trained group was significantly higher than that of the control group (137.60%, P=0.01). Furthermore, caspase-9 gene expression of trained group was significantly higher than that of the control group (48.22%, P=0.01). Conclusion: In general, it seems that a three-month aerobic training was effective in increasing soleus muscle mitochondrial apoptotic protein. However, more research needs to be done to identify the effects of exercise trainings on indices of apoptosis and skeletal muscle atrophy.    }, Keywords = {Aerobic training, Soleus muscle, AIF gene, caspase-9 gene}, volume = {7}, Number = {2}, pages = {257-264}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1143-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1143-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {GholizadeNikpey, Mitra and Mojtahedin, Ali and Seyedsharifi, Rez}, title = {Pain-relieving effects of Silymarin and its interaction with histamine H1 receptors in Rats}, abstract ={Background & Objective: Silymarin is an effective material of Milk thistle whose therapeutic effects have been mentioned in folk medicine. However, little research has been conducted on its antinociceptive effect and its mechanisms of action. Therefore, the aim of present study was to investigate the pain-relieving effects of silymarin using Formalin test with an emphasis on histaminergic system in rats. Materials & Methods: In this experimental study, 42 male rats weighing approximately 200-250 g aged 12-14 weeks were divided into 7 groups with 6 rats in each group: control group (Saline + Formalin 1% intraplantar), 4 treatment groups with silymarin (50, 100, 200 and 400 mg/kg), 1 treatment group with chlorpheniramine (20 mg/kg), 1 pretreatment group with chlorpheniramine (20 mg/kg) + silymarin (200 mg/kg). Formalin test was used to assess somatic pain. One-way ANOVA was used to analyze the data. Results: Intraperitoneal injection of silymarin significantly reduced pain response in both the first and second phase of formalin pain (P<0.05). Pretreatment with chlorpheniramine and then silymarin enhanced the analgesic response in both phases of formalin pain (P<0.05). Conclusion: Silymarin has analgesic effects and these effects are probably caused by using the histaminergic system.      }, Keywords = {Silybum marianum, Pain relief, Silymarin, Histaminergic System, Rat.}, volume = {7}, Number = {2}, pages = {265-274}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1331-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1331-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {BomeAmirhajloo, Abdolkhalegh and AbbasiMaleki, Saei}, title = {Comparison of the anesthesia with Co-administration of Passiflora incarnata hydroalcoholic extract- ketamine with diazepam-ketamine in male rat}, abstract ={Background & Objective: Previous studies have shown analgesic, muscular relaxant, sedative and hypnotic properties of Passiflora incarnata. Hence, in the present study anesthesia with co-administration of Passiflora incarnata-ketamine with diazepam-ketamine was compared. Materials & Methods: In this experimental study, 32 male rats were randomly divided into 3 groups, Passiflora incarnata (PA) (1000mg/kg, i.p.), Passiflora incarnata-ketamine (PIK) (1000mg/kg and 50mg/kg, i.p. respectively) and diazepam-ketamine (DK) (2.5 and 50 mg/kg, i.p. respectively) (n=8). In this research, anesthetic parameters were measured during 0 to 55 minutes. Results: PI group could not induce surgical anesthesia (SA). Nevertheless, PIK group, compared to DK group, immediately induced anesthesia (p>0.05). Results showed that PI group, compared to other groups, increased the heart rate (p<0.05). The respiratory rates of PI and PIK groups increased compared to DK group (p<0.05). The body temperature did not change significantly among groups (p>0.05). PI group became weaker than PIK and DK groups in inhibiting lip, tail and pedal pinches (p<0.05). Conclusion: Induction time, SA and recovery times of PIK group were similar to those of DK group. Although the extract alone could not induce anesthesia, its effects were comparable with diazepam and it could be used as an alternative for a pre-anesthetic agent instead of diazepam.    }, Keywords = { Passiflora incarnata L. extract, ketamine, anesthetic parameters, rat.}, volume = {7}, Number = {2}, pages = {275-282}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1266-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1266-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} } @article{ author = {Sharifat, Narges and Jafari-Hafshejani, Farideh and Dayati, Parisa and Lorestanpoor, Parastoo and Paydar, Ali and BabaahmadiRezaei, Hossei}, title = {Inhibitory effect of Curcumin on phosphorylation NFκB-p65 induced by hydrogen peroxide in Bovine Endothelial Cells}, abstract ={Background & Objective: NFκB is a dimeric transcription factor with multiple subunits. Phosphorylation of  p65 (one of NFκB subunits) by oxidative stress leads to the activation of NFκB. Imbalance between oxidative stress and cellular antioxidant capacity is the pathogenesis of many diseases. Consuming antioxidants in daily meal can be considered as a preventive strategy for inflammatory diseases. Among antioxidant components, curcumin is a natural polyphenol which is extracted from Tumeric. Curcumin can protect the human body from oxidative stress by neutralizing the free radicals. Material & Methods: Bovine Endothelial Cells (BECs) were treated with different concentration of H2O2 (10, 20, 80, 200 μM). To investigate the inhibitory effect of curcumin on H2O2 mediated phosphorylation of p65, BECs were incubated with 5 and 10μM concentration of curcumin. Protein concentration was measured by Bradford method and phosphorylation of p65 was assessed by western blotting. Results: Our finding indicated that p65 phosphorylation was increased two fold in presence of H2O2 (200 μM) in comparison with control. The enhancing effect of H2O2 on p65 phosphorylation decreased at 30 min (P: 0.03) and 2 hours (P:0.015) after treatment with 10 μM dose of curcumin. Conclusion: The result of this study indicates that one of anti-inflammatory mechanisms of curcumin is through NFκB pathway by inhibition of p65 subunit phosphorylation.    }, Keywords = {Curcumin, hydrogen peroxide, transcription factor κB, bovine aortic endothelial cells}, volume = {7}, Number = {2}, pages = {283-290}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1125-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1125-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {2228-5105}, eissn = {2783-1523}, year = {2017} }