:: Volume 5, Issue 1 (Spring 2015) ::
JABS 2015, 5(1): 69-82 Back to browse issues page
In-vitro Differentiation of Human Umbilical Cord Wharton’s Jelly Mesenchymal Stem Cells to Insulin-Producing Cells
Masoomeh Nekohi Madoei 1, Negar Azarpira2, Ladan Sadeghi2, Solmaz Kamali Far3
1- Department of Biology, Islamic Azad University, Arsanjan Branch, Fars, Iran. , masoomnekoei@yahoo.com
2- Organ transplant research center, Namazi hospital, Shiraz University of Medical Sciences, Fars, Iran.
3- Department of Biology, Islamic Azad University, Arsanjan Branch, Fars, Iran.
Abstract:   (12145 Views)


Background & Objective: Diabetes is a major chronic metabolic disease in the world. Islet transplantation is a way to treat diabetes. Unfortunately, this method is restricted due to graft rejection and lack of donor islets. Mesenchymal Stem Cells (MSCS) have the ability to differentiate into Insulin-Producing Cells (IPCs). In this study, Human Umbilical Mesenchymal Stem Cells (HUMSCS) were induced to differentiate into pancreatic β-like cells.


Materials & Methods: The samples were collected after cesarean section delivery at Hafez hospital. HUMSCS were cultured in sterile condition, in three steps for 20 days in DMEM-F12, Retinoic Acid (RA), Epidermal Growth Factor (EGF), exendin-4, Fetal Bovine Serum (FBS), and antibiotic. Then, they were differentiated into IPC. DTZ staining employed for determining the presence of insulin and Reveres Transcription- Polymerase Chain Reaction (RT-PCR) was done for identifying of gene expression including insulin, PDX1, and NGN3. The Insulin concentration was also evaluated by Immunoradiometric assay.


Results: HUMSCS gradually changed from fibroblast-shaped cells to epithelial-like cells and eventually to IPC under special conditions. RT-PCR experiments revealed that these cells expressed insulin, PDX1, and NGN3 genes. The cells became red color when stained with DTZ and the insulin secretion was confirmed.


Conclusion: HUMSCS have the ability to differentiate into islet-like cells in vitro and may be a new potential source for cell transplantation in diabetes treatment.


Keywords: Wharton Jelly, Mesenchymal Stromal Cells, Cell Differentiation, Insulin
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Type of Study: Research | Subject: Cellular-Molecular Biology
Received: 2014/08/18 | Accepted: 2015/01/4 | Published: 2015/06/14

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