Volume 10, Issue 2 (6-2020)
JABS 2020, 10(2): 2338-2347 |
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Alireza Moradabadi1 
, Shariati Aref2 , Taher Azimi3 , Ehsanollah Ghaznavirad4 , Alireza Farsinejad * 5
, Shariati Aref2 , Taher Azimi3 , Ehsanollah Ghaznavirad4 , Alireza Farsinejad * 5
1- Department of Hematology and laboratory medical science, Faculty of Allied Medicine, Kerman University of Medical Science, Kerman, Iran
2- Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
3- Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Department of Microbiology and Immunology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
5- Department of Hematology and laboratory medical science, Faculty of Allied Medicine, Kerman University of Medical Science, Kerman, Iran , Alirezamoradabadi@yahoo.com
2- Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
3- Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Department of Microbiology and Immunology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
5- Department of Hematology and laboratory medical science, Faculty of Allied Medicine, Kerman University of Medical Science, Kerman, Iran , Alirezamoradabadi@yahoo.com
Abstract: (2401 Views)
Background & objective: The aim of this study was to evaluate the therapeutic effect of platelets lysate on leishmaniasis lesions.
Materials & methods: In this study, we used hamster as an animal model and Leishmania major to create an infection in vitro condition. The presence of Leishmania in the site of infection was confirmed by Real-time PCR. We investigated the effect of platelet, plasma, and platelets’ lysate on leishmaniasis lesions and finally, the therapeutic effects of these biomaterials were screened by histological methods.
Results: Real-time PCR method confirmed the presence of parasites in the site of infection. The results of histopathological investigation revealed that in lesions treated with platelet, there were inflammatory response, tissue necrosis and high amount of Leishmania. The wounds extent in both groups treated with plasma and platelet lysate was significantly reduced. However, the result showed that the amounts of Leishmania were significantly reduced in lesions of hamsters treated with platelet lysate.
Conclusion: The results showed that platelet lysate had a better and higher performance than other biomaterials in the treatment and healing of leishmanial lesions.
Materials & methods: In this study, we used hamster as an animal model and Leishmania major to create an infection in vitro condition. The presence of Leishmania in the site of infection was confirmed by Real-time PCR. We investigated the effect of platelet, plasma, and platelets’ lysate on leishmaniasis lesions and finally, the therapeutic effects of these biomaterials were screened by histological methods.
Results: Real-time PCR method confirmed the presence of parasites in the site of infection. The results of histopathological investigation revealed that in lesions treated with platelet, there were inflammatory response, tissue necrosis and high amount of Leishmania. The wounds extent in both groups treated with plasma and platelet lysate was significantly reduced. However, the result showed that the amounts of Leishmania were significantly reduced in lesions of hamsters treated with platelet lysate.
Conclusion: The results showed that platelet lysate had a better and higher performance than other biomaterials in the treatment and healing of leishmanial lesions.
Type of Study: Research |
Subject:
Parasitology
Received: 2019/08/22 | Accepted: 2019/11/3 | Published: 2020/09/19
Received: 2019/08/22 | Accepted: 2019/11/3 | Published: 2020/09/19
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