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:: Volume 10, Issue 2 (Summer 2020) ::
J Fasa Univ Med Sci 2020, 10(2): 2197-2205 Back to browse issues page
Ameliorating Effects of Vitamin E on Morphological and Histological Alterations and Oxidative Stress Factors Assessment Against Titanium Dioxide Nanoparticle-Induced in Mice Testis
Ramin Jahangirfard1 , Ahmad reza Raji 2, Amir Moghaddam jafari3 , Hossein Nourani4
1- Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Department of Comparative Histology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran , rajireza@um.ac.ir
3- Department of Toxicology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
4- Department of Pathology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:   (1071 Views)
Background & Objectives: Nano titanium dioxide particles (TiO2) are chemical compounds that are widely used in industrial and foodstuff such as cosmetics, toothpaste, colored materials, and non-fat milk whitening. Vitamin E (Vit E) is a dietary compound that functions as an antioxidant scavenging free radicals. The goal of this study was to carry out the protective effect of vitamin E on histomorphometry, oxidative stress factors and biochemical parameters as well as testosterone concentration.
Materials & Methods: In the present study, 35 adult male mice were divided into 7 control and experimental groups. The experimental groups received TiO2 at 2.5, 5 and 10 doses, and the positive control group received vitamin E (100 IU) mg/kg alone (by gavage tube). After treatment, animals were euthanized by cervical dislocation and testes samples were taken. The level of p<0.05 was considered significant.
Results: The results of histomorphometry revealed that the weight of testis and body, testicular capsule thickness, seminiferous tubules diameter, germinal epithelium height, spermiogenesis and meiotic indices were reduced while sertoli cells and repopulation index had no significant changes. Also, biochemical results showed the Malondialdehyde was enhanced and it diminished the total antioxidant capacity, catalase enzyme activity, protein content, as well as testosterone concentration. (p<0.05).
Conclusion: The TiO2 nanoparticles caused detrimental pathologic effects when used at the highest doses. However, vitamin E could ameliorate the toxic effects of TiO2-induced on testicular tissue in experimental animals.
 
 
Keywords: Histomorphometry, Mice, Testis, Titanium dioxide, Vitamin E
Full-Text [PDF 902 kb]   (245 Downloads)    
Type of Study: Research | Subject: Histology
Received: 2020/05/15 | Accepted: 2020/07/7 | Published: 2020/09/19
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jahangirfard R, raji A R, moghaddam jafari A, Nourani H. Ameliorating Effects of Vitamin E on Morphological and Histological Alterations and Oxidative Stress Factors Assessment Against Titanium Dioxide Nanoparticle-Induced in Mice Testis. J Fasa Univ Med Sci. 2020; 10 (2) :2197-2205
URL: http://jabs.fums.ac.ir/article-1-2330-en.html


Volume 10, Issue 2 (Summer 2020) Back to browse issues page
Journal of Fasa University of Medical Sciences
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