:: Volume 10, Issue 3 (Atumn 2020) ::
J Fasa Univ Med Sci 2020, 10(3): 2429-2438 Back to browse issues page
In Silico Prediction of Continuous Epitopes on Human immunoglobulin Light Chains
Soheyla Rohani1 , Fatemeh Hajighasemi 2
1- Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran
2- Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran , fatimahajighasemi@gmail.com
Abstract:   (950 Views)
Background & Objective: Immunoglobulins (Igs) have a vital role in body protection against germs. Igs contain heavy and light chains. Ig light chains consist of two isotypes: Kappa and lambda. Remarkable alterations in kappa to lambda proportion could occur in monoclonal growth of malignant B cells. Anti-human light chain monoclonal antibodies (MAbs) have medical implication in diagnosis and immunotherapy of B-cell proliferative disorders. In current study, prediction of continuous epitopes in constant part of human Ig light chain by immunoinformatics is explained.
Materials & Methods: Amino acid sequence and third structure of reference human IgG light chain was obtained in PDB database. Second IgG structure was identified by Phyre 2 software. Continuous epitopes of Ig light chains were delineated by Bepipred and Ellipro software programs.
Results: Four continuous epitopes situated to constant domain of human Ig light chain were predicted by Bepipred software. These continuous epitopes were located at amino acid sequences 110-130, 150-160, 160-175 and 180-205 of Ig light chain. The prominent epitope was sited at amino acids 160-175. Also, one continuous epitope situated in 198-203 amino acid sequences was predicted by Ellipro software.
Conclusion: In present study several continuous epitopes sited to constant part of human Ig light chain were determined. These epitopes are valuable for making specific monoclonal anti- Ig light chain antibodies and could have plausible implication in generation of specific diagnostic kits for human Ig light chain, monitoring the monoclonal light chain diseases, treatment of associated B cell malignancies and epitope mapping of Ig light chain.
 
 
Keywords: Continuous epitope, Immunoglobulins, Light chains, Immunoinformatics
Full-Text [PDF 948 kb]   (176 Downloads)    
Type of Study: Research | Subject: Immunology
Received: 2020/06/14 | Accepted: 2020/08/10 | Published: 2021/01/26


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Volume 10, Issue 3 (Atumn 2020) Back to browse issues page