:: Volume 11, Issue 3 (Atumn 2021) ::
JABS 2021, 11(3): 3993-4004 Back to browse issues page
Novel Combination of Periodontal Ligament Stem Cells (PDLSCs)-Conditioned Medium and Taurine Increase Apoptosis in U87Glioma Tumor Cells: Possible Relevance to Glioblastoma Treatment
Masoumeh Einabadi1 , Jafar Ai 2, Hooshang Jamali3 , Mohammad Kargar1 , Farshid Kafilzadeh1
1- Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
2- Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Immunology and Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Abstract:   (596 Views)
Background & Objective: Glioblastoma Multiforme is a very aggressive primary brain cancer that has a median overall survival even if the treatment period is less than 1 year. Despite progress in glioblastoma diagnosis and treatment, the prognosis of patients with glioblastoma still remains poor. Recently, it has been detected that stem cell therapies are an effective method for brain tumor cell targeting. Also, the anti-inflammatory and anti-apoptotic activity of taurine against cancerous cells has been found.
Materials & Methods: In this study, we employed taurine as an anti-cancer drug and PDLSCs as a potential agent for improving anti-cancer drug efficiency. Then, we investigated their effect on the glioma tumor cells in in-vitro 2D cell culture and in vivo.
Results: Taurine had the best apoptotic activity on the C6 glioblastoma cells at the concentration of 80 μM after 72h post-treatment. The obtained results showed that a combination of taurine/PDLSCs induced the expression of caspase-3, caspase-8, and IL-17Ra and down-regulated the expression of Bcl-2 and IL-17Ra. Uses of taurine and PDLSCs suppress the migration of the cancerous C6 cells after 48h and show good potential in the suppression of GBM metastasis.
Conclusion: The taurine at concentration of 80µM has a strong potential in decreasing the viability of cancerous C6 Glioblastoma cells. Therefore, taurine and PDLSCs combination, due its therapeutic efficacy, has a considerable potential to be a successful method to glioblastoma brain cancer treatment.
Keywords: Glioblastoma Multiform, Taurine, Periodontal Ligament Stem Cells, Apoptosis
Full-Text [PDF 541 kb]   (37 Downloads)    
Type of Study: Research | Subject: Oncology
Received: 2021/05/29 | Accepted: 2021/06/9 | Published: 2021/11/13

XML   Persian Abstract   Print

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 11, Issue 3 (Atumn 2021) Back to browse issues page