Background & Objective: Urinary tract infection is one of the most common bacterial infections in humans and its appropriate treatment is extremely important. Occurrence of antibiotic resistance in bacterial pathogen is a worldwide problem. The evaluation of bacterial susceptibility pattern and recognizing the prevalent resistances can be helpful for appropriate treatment and management. The objective of the present study is to evaluate antibiotic resistance in gram-negative bacteria isolates of urine cultures of patients hospitalized in Fasa Vali-Asr hospital during the years 2012 and 2014. .

Materials & Methods: In this cross-sectional descriptive study, urine samples of hospitalized patients in different wards of Vali-Asr hospital of Fasa were sent to the laboratory. After identification of strains, the antibiotic sensitivity definition test, using Antibiogram disk diffusionmethod, was done.

Results: In this study, UTI was caused bygram negative bacteria. Most bacterial isolates showed highest resistance rate to ampicillin and lowest resistance rate to amikacin.

Conclusion: In this study, Amikacin and Cefotaxime were identified as the most effective drugs to treat the majority of patients with urinary tract infections. However, according to Antibiogram results in different geographical regions, the use of region antibiotic resistance pattern is essential in patients’ treatment.