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:: Volume 5, Issue 4 (Winter 2016) ::
J Fasa Univ Med Sci 2016, 5(4): 501-507 Back to browse issues page
Outer Membrane Protein D Gene in Clinical Isolates of Pseudomonas Aeruginosa and its Role in Antibiotic Resistance
Badrosadat Motaghi1, Sohrab Najafipour 2
1- Department of Microbiology, College of Microbiology, Shiraz Science and Research Branch, Islamic Azad University, Shiraz, Iran.
2- Department of Microbiology, Fasa Medical School, Fasa University of Medical Sciences, Fasa. Iran. , sohrabnajafipour@yahoo.com
Abstract:   (10856 Views)

Background & Objectives: Pseudomonas aeruginosa is a common cause of nosocomial infection. OprD protein is a specific protein regulating the uptake of carbapenem antibiotic. Loss of OprD is the main mechanism of Pseudomonas Aeruginosa resistance to carbapenem. In this study, the presence of OprD gene is investigated in isolated Pseudomonas Aeruginosa in burn patients of Ghotboddin hospital in Shiraz.

Material & Methods: 66 Pseudomonas Aeruginosa were isolated from wound specimens of 250 burn patients. Strain characteristics were confirmed by biochemical tests. Antibiogram was done via disc diffusion method. Finally, OprD gene was investigated by PCR.

Results: Isolated Pseudomonas Aeruginosa showed more sensitivity to chloramphenicol and colicitin and more resistance  to ciprofloxacin, gentamycin, cefotaxim, ceftazidin, imipenem, meropenem, and erythromycin. 61 percent of isolates were positive for OprD gene by PCR.

Conclusion: The findings of this study revealed that Colicitin and chloramphenicol are more effective in treatment of Pseudomonas Aeruginosa infections in burn patients, and deletion and mutation in OprD gene cause bacterium resistance to carbapenem antibiotic.

Keywords: KeyWords: OprD, Pseudomonas aeruginosa, Antibiotic Resistance, PCR
Full-Text [PDF 714 kb]   (3066 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2015/08/10 | Accepted: 2015/12/16 | Published: 2016/02/27
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Motaghi B, Najafipour S. Outer Membrane Protein D Gene in Clinical Isolates of Pseudomonas Aeruginosa and its Role in Antibiotic Resistance. J Fasa Univ Med Sci. 2016; 5 (4) :501-507
URL: http://jabs.fums.ac.ir/article-1-885-en.html

Volume 5, Issue 4 (Winter 2016) Back to browse issues page
Journal of Fasa University of Medical Sciences
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