@ARTICLE{Zargar, author = {Morovvati, Abbas and Javadi, Ali and Dehghani sanij, Somayeh and Zarei, Mohamadreza and Molazadeh, Shima and Badakhsh, Mina and Zargar, Mohsen and }, title = {Rapid Homemade Reverse Transcriptase PCR Detection and Phylogenetic Analysis for SARS-CoV-2 Based on E and M Genes}, volume = {12}, number = {2}, abstract ={Background & Objective: Coronavirus disease 2019 spreads worldwide and needs detection systems capable of rapid diagnostic of this virus (SARS-CoV-2). The aim of this study is to design the homemade RT-PCR method for the Detection and phylogenetic analysis of this virus Material & Methods: The genes selected for diagnosis were E and M genes for this virus. PCR product was cloned in pTZ57R/T plasmid for preparation of positive control. In order to determine the sensitivity of this molecular method, the genes mentioned in the clone pTZ57R/T vector and the Limit of detection (LOD) the genes were determined and phylogenetic analysis was performed using partial E and M gene sequences. Results: PCR product was observed for E and M genes 156 and 547 bp on the Agarose gel. The LOD of the E and M gene was 60 and 82 copies. There was also a positive response to the samples of patients who were positive by other methods. Conclusions: Since this virus is considered to be the cause of a pandemic in different countries all over the world, the present study is very important as a method of rapid and low-cost molecular diagnosis for monitoring this virus. Phylogenetic analysis is necessary for epidemiological studies for the control and prevention of the disease. }, URL = {http://jabs.fums.ac.ir/article-1-2773-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2773-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, doi = {10.18502/jabs.v12i2.9883}, year = {2022} }