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:: Volume 9, Issue 4 (Winter 2019) ::
J Fasa Univ Med Sci 2019, 9(4): 1710-1718 Back to browse issues page
The Effect of Endurance Training and L-Carnitine Supplementation on Gene Expression of Hepatic Enzymes (AST,ALT,ALP) in Wistar Male Rats Toxicated by Boldenone
Pooneh Pashayi1 , Asieh Abbassi Daloii 2, Ali Reza Barari1 , Syed Reza Mir Javadi3
1- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , abbasi.daloii@gmail.com
3- Department of Sport Physiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract:   (948 Views)
Background & Objective: The aim of this study was to investigate the effect of endurance training and L-carnitine supplementation on gene expression of hepatic enzymes (AST, ALT, ALP) in Wistar male rats toxicated by Boldenone.
Materials & Methods: In this experimental study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, no-treatment, boldenone (5mg/kg), L-carnitine and aerobic training- L-carnitine. The endurance moderate intensity training program (50-55% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. Boldenone injection once a week, on an appointed day, and in the hamstring was conducted in depth. After anesthesia, autopsy was performed and the liver was isolated. The hepatic enzymes gene expression in the samples were measured by Real Time PCR. Data were analyzed by t-test, One-way ANOVA and post hoc Scheffe at the significant level P<0.05.
Results: The results showed that there was a significant difference between the mean expression of liver enzymes (AST, ALT, ALP) in male Wistar rats in different groups (P<0.001). The changes in liver enzymes gene expression (AST, ALT, ALP) in L-carnitine and Training-L-carnitine groups were significantly lower than the boldenone group (P<0.001).
Conclusion: According to the findings of this study, supplementation of L-carnitine with regular aerobic training reduces liver damage induced by anabolic androgenic steroids.
 
Keywords: 
Keywords: Aerobic training, Boldenone, L-carnitine, Hepatic enzymes, Wistar rats
Full-Text [PDF 1032 kb]   (136 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2019/09/15 | Accepted: 2019/12/7 | Published: 2020/03/15
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Pashayi P, Abbassi Daloii A, Barari A R, Mir Javadi S R. The Effect of Endurance Training and L-Carnitine Supplementation on Gene Expression of Hepatic Enzymes (AST,ALT,ALP) in Wistar Male Rats Toxicated by Boldenone. J Fasa Univ Med Sci. 2019; 9 (4) :1710-1718
URL: http://journal.fums.ac.ir/article-1-2121-en.html


Volume 9, Issue 4 (Winter 2019) Back to browse issues page
Journal of Fasa University of Medical Sciences
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