Journal of Advanced Biomedical Sciences
JABS
Sun, Jan 5, 2025
[Archive]
Volume 9, Issue 4 (11-2019)
JABS 2019, 9(4): 1710-1718 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Pashayi P, Abbassi Daloii A, Barari A R, Mir Javadi S R. The Effect of Endurance Training and L-Carnitine Supplementation on Gene Expression of Hepatic Enzymes (AST,ALT,ALP) in Wistar Male Rats Toxicated by Boldenone. JABS 2019; 9 (4) :1710-1718
URL: http://jabs.fums.ac.ir/article-1-2121-en.html
URL: http://jabs.fums.ac.ir/article-1-2121-en.html
1- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,abbasi.daloii@gmail.com
3- Department of Sport Physiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,
3- Department of Sport Physiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract: (4142 Views)
Background & Objective: The aim of this study was to investigate the effect of endurance training and L-carnitine supplementation on gene expression of hepatic enzymes (AST, ALT, ALP) in Wistar male rats toxicated by Boldenone.
Materials & Methods: In this experimental study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, no-treatment, boldenone (5mg/kg), L-carnitine and aerobic training- L-carnitine. The endurance moderate intensity training program (50-55% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. Boldenone injection once a week, on an appointed day, and in the hamstring was conducted in depth. After anesthesia, autopsy was performed and the liver was isolated. The hepatic enzymes gene expression in the samples were measured by Real Time PCR. Data were analyzed by t-test, One-way ANOVA and post hoc Scheffe at the significant level P<0.05.
Results: The results showed that there was a significant difference between the mean expression of liver enzymes (AST, ALT, ALP) in male Wistar rats in different groups (P<0.001). The changes in liver enzymes gene expression (AST, ALT, ALP) in L-carnitine and Training-L-carnitine groups were significantly lower than the boldenone group (P<0.001).
Conclusion: According to the findings of this study, supplementation of L-carnitine with regular aerobic training reduces liver damage induced by anabolic androgenic steroids.
Keywords:
Materials & Methods: In this experimental study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, no-treatment, boldenone (5mg/kg), L-carnitine and aerobic training- L-carnitine. The endurance moderate intensity training program (50-55% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. Boldenone injection once a week, on an appointed day, and in the hamstring was conducted in depth. After anesthesia, autopsy was performed and the liver was isolated. The hepatic enzymes gene expression in the samples were measured by Real Time PCR. Data were analyzed by t-test, One-way ANOVA and post hoc Scheffe at the significant level P<0.05.
Results: The results showed that there was a significant difference between the mean expression of liver enzymes (AST, ALT, ALP) in male Wistar rats in different groups (P<0.001). The changes in liver enzymes gene expression (AST, ALT, ALP) in L-carnitine and Training-L-carnitine groups were significantly lower than the boldenone group (P<0.001).
Conclusion: According to the findings of this study, supplementation of L-carnitine with regular aerobic training reduces liver damage induced by anabolic androgenic steroids.
Keywords:
Type of Study: Research |
Subject:
Physiology
Received: 2019/09/15 | Accepted: 2019/12/7 | Published: 2020/03/15
Received: 2019/09/15 | Accepted: 2019/12/7 | Published: 2020/03/15
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)