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:: Volume 6, Number 2 (Summer 2016) ::
J Fasa Univ Med Sci 2016, 6(2): 235-245 Back to browse issues page
Evaluating Superoxide Dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA) and the Histological Changes of the Lung Tissue after γ-Irradiation in Rats
Abolhasan Rezaeyan1, Gholam Hassan Haddadi 2, Massood Hosseinzadeh3
1- MSc Department of Medical Physics, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
2- Associate Professor of Medical Physics Department of Medical Physics, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran , ghadadi@gmail.com
3- Associate Professor of Pathology Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (3179 Views)

Background & Objective: The lung is a radiosensitive organ and its damage is a dose-limiting factor in radiotherapy. Different side effects such as pneumonia and lung fibrosis are found in patients with thorax irradiation. The objective of the present study is to evaluate the effects of γ-irradiation on acute and chronic injuries of lung tissue in rats.

Materials & Methods: 32 rats were divided into two groups. Control group consisted of 14 rats that underwent shame irradiation. In radiation group, 18 rats underwent γ-irradiation. The rats were exposed to γ-irradiation 18 Gy using a single fraction cobalt-60 unit. Eight rats in each group were sacrificed 24 hours after radiotherapy for determining Superoxide Dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA), and histopathological evaluations. Remained animals were sacrificed eight weeks after radiotherapy for histopathological evaluation.

Results: Compared to control group, the level of SOD and GSH significantly decreased and MDA level significantly increased in radiation group 24 hours following irradiation, (p=0.001, p<0.001, p=0.001) respectively. Early histopathological results after 24 hours showed that radiation increases neutrophil, macrophage, and inflammation incidence compared to control group (p<0.05). Late histopathological evaluation after eight weeks revealed significant increase in factors including mast cells, pulmonary edema, vascular thickness, vascular damage, and also inflammation and fibrosis incidence in case group compared to radiation group

 (p<0.05).

Conclusion: Localized chest radiation with dose of 18 Gy induces changes in oxidative stress indices and histopathological lung tissue damage in short and long term.

Keywords: Superoxide Dismutase, Glutathione, Malondialdehyde, Histopathology, Gamma-radiation
Full-Text [PDF 1430 kb]   (1427 Downloads)    
Type of Study: Research | Subject: Medical Physics
Received: 2016/01/22 | Accepted: 2016/06/5 | Published: 2016/09/10
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Rezaeyan A, Haddadi G H, Hosseinzadeh M. Evaluating Superoxide Dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA) and the Histological Changes of the Lung Tissue after γ-Irradiation in Rats. J Fasa Univ Med Sci. 2016; 6 (2) :235-245
URL: http://journal.fums.ac.ir/article-1-1007-en.html
Volume 6, Number 2 (Summer 2016) Back to browse issues page
Journal of Fasa University of Medical Sciences
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