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:: Volume 6, Number 4 (Winter 2017) ::
J Fasa Univ Med Sci 2017, 6(4): 474-480 Back to browse issues page
Evaluation of Serum Specific Antibody against Recombinant ESAT-6 Antigen in Patients with Tuberculosis and Comparing to Normal Controls
Homeira Izadi1, Nooshin Sohrabi 2, Majid Tebianian3, Nader Mosavari3, Mehdi Mahdavi4
1- Department of Biology, Payame Noor University, Tehran, Iran
2- Department of Biology, Payame Noor University, Tehran, Iran , nsohrabi75@yahoo.com
3- Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4- Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (1746 Views)

Background & Objective: Tuberculosis (TB) is a zoonotic disease which is caused by Mycobacterium tuberculosis. Because of common structural and secretory antigens between pathogen and nonpathogenic mycobacterium, the specific diagnosis of TB is difficult. Therefore, it is very important to find a new method with high specificity and sensitivity for accurate and rapid diagnosis of tuberculosis. In this study, the serodiagnostic potential of Mycobacterium tuberculosis recombinant ESAT-6 in TB infected patients was evaluated by Enzyme Linked Immunosorbent Assay (ELISA).

Materials & Methods: 55 TB patients with active disease and 28 healthy controls have been collected and evaluated in different dilutions in ELISA methods for the presence of specific anti-ESAT-6 antibody. The specificity and the sensitivity of this method was compared with the culture test.

Results: TB patients have high levels of specific antibody against ESAT-6 antigens. The specificity and the sensitivity of this method was calculated as 80.90% and 85.45%, respectively.

Conclusion: These findings provide useful information on the importance of ESAT-6 protein and suggested this serologic test as a good alternative method for rapid and prefect diagnosis of tuberculosis.

Keywords: Mycobacterium tuberculosis, ESAT-6, Antibody, ELISA
Full-Text [PDF 1276 kb]   (449 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2016/06/18 | Accepted: 2016/11/1 | Published: 2017/03/8
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Izadi H, Sohrabi N, Tebianian M, Mosavari N, Mahdavi M. Evaluation of Serum Specific Antibody against Recombinant ESAT-6 Antigen in Patients with Tuberculosis and Comparing to Normal Controls. J Fasa Univ Med Sci. 2017; 6 (4) :474-480
URL: http://journal.fums.ac.ir/article-1-1147-en.html
Volume 6, Number 4 (Winter 2017) Back to browse issues page
Journal of Fasa University of Medical Sciences
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