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:: Volume 7, Issue 4 (winter 2018) ::
J Fasa Univ Med Sci 2018, 7(4): 447-454 Back to browse issues page
The phytoestrogenic effect of hydroalcoholic extract of alfalfa on oxidative stress and follicle formation in adult female Wistar rats
Sajad Sistani 1, Mahdieh Raeeszadeh 2, Aliakbar Amiri 3
1- Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2- Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , vet_mr@yahoo.com
3- Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Abstract:   (1881 Views)

Background & Objectives: Today, Infertility problems including lack of follicle formation, among other things, are of paramount importance. The aim of this study was to investigate the effect of hydroalcoholic extract of alfalfa on oxidative stress and ovarian tissue in female rats.
Materials & Methods: In this experimental-interventional study, 24 rats weighing 200-250g were randomly divided into three groups: control (no treatment) and experimental groups (T1 and T2) which received alfalfa extract at 25 and 50mg/kg intraperitoneally for 25 days, respectively. Body weight of animals and their ovarian weight were measured. The left ovary was prepared for histological study. The antioxidant capacity in serum and malondialdehyde in right ovary extracts were also measured.
Results: At the end of the study, the body weight of the animals in both control and experimental groups compared to that of pre-intervention time, but the increase was not statistically significant (P>0.05). The most ovarian weight was observed in T2group (160g) and the lowest weight was in the control group (146g). The primary and secondary follicles and corpus luteum were the highest number in the T2group of alfalfa extract and the lowest number in the control group. The serum antioxidant capacity in T1 and T2groups were about 922 and 937µmol/ml, respectively, and the lowest amount was in the control group with 780µmol/ml and the difference was significant. Malondialdehyde concentration in control and T2gruop were the average of 0.35 and 0.22µmol/ml, respectively (P<0.05).
Conclusion: Hydroalcoholic extract of alfalfa with phytoestrogenic and oxidative stress control effects can enhance ovarian activity in adult rats.
 

Keywords: Hydroalcoholic extract of alfalfa, ovary, oxidative stress, Wistar rat
Full-Text [PDF 374 kb]   (312 Downloads)    
Type of Study: Research | Subject: Pharmacology
Received: 2016/11/20 | Accepted: 2017/07/6 | Published: 2017/11/26
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Sistani S, Raeeszadeh M, Amiri A. The phytoestrogenic effect of hydroalcoholic extract of alfalfa on oxidative stress and follicle formation in adult female Wistar rats. J Fasa Univ Med Sci. 2018; 7 (4) :447-454
URL: http://journal.fums.ac.ir/article-1-1209-en.html


Volume 7, Issue 4 (winter 2018) Back to browse issues page
Journal of Fasa University of Medical Sciences
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