Volume 10, Issue 3 (8-2020)                   JABS 2020, 10(3): 2578-2587 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

moradozadeh G, zia jahromi N, sazgar H. Evaluation of Cell Biomarker Gene Expression (CD34) in ALL Patients and Healthy Controls Expression (CD34) in People with Leukemia. JABS 2020; 10 (3) :2578-2587
URL: http://jabs.fums.ac.ir/article-1-1999-en.html
1- Department of Biology, Faculty of Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Department of Biology, Faculty of Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , nooshaziajahromi.59@gmail.com
Abstract:   (2155 Views)
Background & Objective: CD34 is a prognostic factor in determining the prognosis of patients with acute myeloid leukemia. Since the relationship between expression of the marker of the CD34 cell line and many cancers has been proven today, in this study, the measurement of the expression of this marker with leukemia was investigated.
Materials & methods: The present study is a case-control study performed on 30 healthy blood samples and 30 blood samples from blood donors. Lymphocytes were isolated using Ficole method. RNA was extracted. After synthesis of cDNA, expression regions were determined using Real Time RT-PCR with an internal control of GAPDH.
Results: After performing the PCR reaction with specific primers in Real Time RT PCR and analyzing the results, the expression of CD34 gene in the blood of cancer patients was increased compared to healthy subjects (F = 21.588, P-Val = 0.002).
Conclusion: Finding new biomarkers, which are a common indicator of cancer, can help with early diagnosis and recovery during cancerous illnesses. By reviewing these biomarkers, it is possible to develop new anti-cancer drugs that can target cancerous cells and prevent growth from growing into cancerous cells.
 
 
Full-Text [PDF 1257 kb]   (963 Downloads)    
Type of Study: Research | Subject: Cellular-Molecular Biology
Received: 2019/04/9 | Accepted: 2020/05/23 | Published: 2021/01/26

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)