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:: Volume 12, Issue 2 (Spring(2022) 2022) ::
JABS 2022, 12(2): 203-214 Back to browse issues page
Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain
Marjan Seratnahaei 1, Seyyed Saeed Eshraghi2 , Parviz Pakzad3 , Alireza Zahraei Ramazani4 , Mehdi Yaseri5
1- Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran , Marjan.nahaee@gmail.com
2- Department of Pathobiology, Faculty of Public Health, Tehran University of Medical Science, Tehran, Iran
3- Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
4- Department of Medical Entomology and Vector Control, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Epidemiology and Biostatistics, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (1510 Views)
Background & Objective: The emergence of antibiotic-resistant bacteria and limited treatment options for these infections is a challenging problem in the world. There is increasing interest in isolating bioactive molecules from actinobacteria and elucidating their antimicrobial mechanisms at the moment. The current study aimed to investigate the antimicrobial activities of a Nocardia strain (N4) that was previously isolated from the soil samples in Tehran, Iran.
 Materials & Methods: The cultural, morphological, and physiological characteristics of the strain were determined and the identity of the strain was confirmed using 16SrRNA gene sequencing. The strain was screened for antimicrobial metabolite production. The in vitro anti-microbial activity of the extracted metabolite was assessed by agar well diffusion and minimum inhibitory concentrations methods. Finally, the structure of the extracted metabolite was confirmed by HPLC and mass spectrometry methods.
Results: The isolate shared 99% sequence similarity with Nocardia soli. The strain showed broad-spectrum antimicrobial activities against some pathogenic bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603, Salmonella Typhimurium ATCC 14028 and Shigella sonnei RI366) and fungi (Aspergillus niger ATCC 1015 and Aspergillus fumigatus ATCC 1022) in the agar well diffusion method. The ethyl acetate extract of the strain exhibited the highest antimicrobial activity against E. coli (MIC= 4.67±3.055 μg/mL) and K. pneumoniae (MIC=3.33±1.155 μg/mL). The metabolite showed high similarity with C21H28N7O14.
Conclusions: The current study confirmed the presence of a bioactive metabolite in strain N4, suggesting that this may be a promising candidate for discovering bioactive components with broad antibacterial activities against a variety of pathogens

Keywords: 
Keywords: Nocardia soli, antimicrobial activity, HPLC, Bioactive Metabolite
Full-Text [PDF 462 kb]   (48 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2021/07/26 | Accepted: 2022/03/8 | Published: 2022/06/28
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Seratnahaei M, Eshraghi S S, Pakzad P, Zahraei Ramazani A, Yaseri M. Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain. JABS. 2022; 12 (2) :203-214
URL: http://jabs.fums.ac.ir/article-1-2700-en.html


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Volume 12, Issue 2 (Spring(2022) 2022) Back to browse issues page
Journal of Advanced Biomedical Sciences
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