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:: Volume 12, Issue 2 (Spring(2022) 2022) ::
JABS 2022, 12(2): 215-222 Back to browse issues page
Evaluation of LncRNA uc.173 and Occludin in Iranian Patients with Inflammatory Bowel Disease Compared with Healthy Individuals
Vahid Chaleshi1 , Saeideh Oloumi Kalantar2 , Iman Salahshourifar2 , Shabnam Shahrokh1 , Hamid Asadzadeh Aghdaei 3, Mohammad Reza Zali4
1- Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran , hamid.assadzadeh@gmail.com
4- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:   (872 Views)
Background & Objective: Inflammatory bowel disease (IBD) is characterized by the chronic gastrointestinal inflammation. The two common forms of IBD are ulcerative colitis (UC) and Crohn’s disease (CD) that are distinguished by their location and depth of involvement in the diffuse inflammation of the colonic mucosa and affects the rectum (proctitis). A novel class of LncRNAs transcribed from ultra-conserved regions (UCRs) is a recently identified ultra-conserved region (T-UCR) transcript that is involved in the cellular function in a variety of pathways. However, the regulation of LncRNA uc.173 in IBD remains to be fully elucidated. In this study, we aimed to examine the expression of LncRNA uc.173 and Occludin genes in an Iranian population with inflammatory bowel disease.
Materials & Methods: This case-control study was performed on 33 inflammatory bowel disease patients including 13 Crohn's disease, 20 ulcerative colitis and 20 healthy controls. The mRNA levels of LncRNA uc.173 and Occludin genes were assessed using the quantitative Real-time polymerase chain reaction. The B2M was used as an internal control. The 2 -ΔΔCq method was used to determine the expression fold changes.
Results: Statistically, the level of the LncRNA uc.173 gene expression between the UC and normal tissues increased significantly(P=0.0024). Also, the expression analysis revealed no significant difference between the samples of CD patients compared to the controls (P>0.05). In order to further evaluate the role of LncRNA uc.173 in IBD, the associations between the transcript levels of the LncRNA uc.173 and Occludin mRNA demonstrated significant difference in the CD tissue (R=0.59; P=0.002). In our study, the mRNA expression of Occludin gene did not show any changes in the IBD patients compared to the healthy controls.
Conclusion: The increased expression of LncRNA uc.173 in the tissues of UC patients may be considered as a diagnostic or prognostic biomarker. Also, there was no correlation found between Occludin and LncRNA uc.173 expressions in the IBD patients' tissues.
 
Keywords: Inflammatory bowel disease, Ulcerative colitis, Crohn's disease, LncRNA uc.173, Occludin
Full-Text [PDF 557 kb]   (92 Downloads)    
Type of Study: Research | Subject: Cellular-Molecular Biology
Received: 2021/12/20 | Accepted: 2022/03/13 | Published: 2022/06/28
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Chaleshi V, Oloumi Kalantar S, Salahshourifar I, Shahrokh S, Asadzadeh Aghdaei H, Zali M R. Evaluation of LncRNA uc.173 and Occludin in Iranian Patients with Inflammatory Bowel Disease Compared with Healthy Individuals. JABS 2022; 12 (2) :215-222
URL: http://jabs.fums.ac.ir/article-1-2770-en.html


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Volume 12, Issue 2 (Spring(2022) 2022) Back to browse issues page
Journal of Advanced Biomedical Sciences
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