Journal of Advanced Biomedical Sciences
JABS
Wed, Apr 24, 2024
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Volume 13, Issue 1 (3-2023)
JABS 2023, 13(1): 81-87 |
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Dalir F, Morovvati A, Javadi A, Afkhami H, Khaledi M, Fathi J, et al . The Evaluation of Efflux Pump Genes norA, norB and norC Related to Fluoroquinolones Resistance in Staphylococcus aureus Strains Isolated from Blood Infection. JABS 2023; 13 (1) :81-87
URL: http://jabs.fums.ac.ir/article-1-2910-en.html
URL: http://jabs.fums.ac.ir/article-1-2910-en.html
Fateme Dalir1 
, Abbas Morovvati1 , Ali Javadi2 , Hamed Afkhami3 , Mansoor Khaledi3 , Javad Fathi4 , Zeinab Mohsenipour5 , Ashkan Dirbaziyan1 , Mohsen Zargar * 1
, Abbas Morovvati1 , Ali Javadi2 , Hamed Afkhami3 , Mansoor Khaledi3 , Javad Fathi4 , Zeinab Mohsenipour5 , Ashkan Dirbaziyan1 , Mohsen Zargar * 1
1- Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
2- Department of Medical Science, Qom Branch, Islamic Azad University, Qom, Iran
3- Department of Microbiology, School of Medicine, Shahed University, Tehran, Iran
4- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5- Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2- Department of Medical Science, Qom Branch, Islamic Azad University, Qom, Iran
3- Department of Microbiology, School of Medicine, Shahed University, Tehran, Iran
4- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5- Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract: (594 Views)
Background & Objective: Recently, ciprofloxacin resistance in Staphylococcus aureus strains, due to efflux pumps, has become a significant challenge. Therefore, this study was performed to evaluate the frequency of norA, norB, and norC efflux pump genes and their roles in resistance to ciprofloxacin in clinical isolates of S. aureus.
Materials & Methods: A total of one hundred clinical blood samples were collected from patient in Qom hospitals and S. aureus isolates were identified by standard microbiological tests. Antimicrobial susceptibility patterns were determined by the disk diffusion method using CLSI guidelines. Subsequently, the presence of norA, norB, and norC efflux pump genes in ciprofloxacin isolates was detected using the PCR method.
Results: Among one hundred clinical samples, 36 S. aureus isolates were recovered and the results of antibiotic susceptibility tests showed that twenty of them were resistant to ciprofloxacin. 15 isolates were resistant to norfloxacin and one isolate was resistant to ofloxacin. Moreover, the norA, norB, and norC genes were found in 58%, 30%, and 41% of ciprofloxacin-resistant isolates, respectively.
Conclusion: Based on the results of this study, norA, norB, and norC efflux pumps may play a significant role in the development of resistance to ciprofloxacin in clinical isolates of S. aureus. Detecting these genes may prove useful in suggesting an effective treatment model for infections caused by S. aureus.
Materials & Methods: A total of one hundred clinical blood samples were collected from patient in Qom hospitals and S. aureus isolates were identified by standard microbiological tests. Antimicrobial susceptibility patterns were determined by the disk diffusion method using CLSI guidelines. Subsequently, the presence of norA, norB, and norC efflux pump genes in ciprofloxacin isolates was detected using the PCR method.
Results: Among one hundred clinical samples, 36 S. aureus isolates were recovered and the results of antibiotic susceptibility tests showed that twenty of them were resistant to ciprofloxacin. 15 isolates were resistant to norfloxacin and one isolate was resistant to ofloxacin. Moreover, the norA, norB, and norC genes were found in 58%, 30%, and 41% of ciprofloxacin-resistant isolates, respectively.
Conclusion: Based on the results of this study, norA, norB, and norC efflux pumps may play a significant role in the development of resistance to ciprofloxacin in clinical isolates of S. aureus. Detecting these genes may prove useful in suggesting an effective treatment model for infections caused by S. aureus.
Type of Study: Research |
Subject:
Microbiology
Received: 2022/10/17 | Accepted: 2023/04/3 | Published: 2023/04/24
Received: 2022/10/17 | Accepted: 2023/04/3 | Published: 2023/04/24
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This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)