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:: Volume 5, Number 1 (Spring 2015) ::
J Fasa Univ Med Sci 2015, 5(1): 134-141 Back to browse issues page
Evaluation of Antibacterial Effect of Curcumin Loaded Chitosan Nanoparticles
Mirza Ali Mofazzal Jahromi1, Hajar Rajayi2, Sharafaldin Al-Musawi3, Majid Pirestani4, Mahdi Fasihi Ramandi1, Kazem Ahmadi1, Vahid Sharifzadeh Peivasti5, Zuhair Mohammad Hassan2, Mahdi Kamali6, Reza Mirnejad 7
1- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2- Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
3- Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
4- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
5- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
6- Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
7- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. , rmirnejad@yahoo.com
Abstract:   (4888 Views)

 

Background & Objective: Nanomedicine delivery systems are known as potent therapeutictools. In addition to possessing an effective agent of turmeric, Curcumin shows antibacterial properties as well. Curcumin is not water-soluble and it can be solved in water via nanotechnology-base methods. Chitosan is a natural and biodegradable substance that is utilized for the Production of Nanoparticles (NPs) carrying drug. In the following investigation, curcumin is loaded in chitosan NPs and ultimately, the resulting nano-drug is studied as an antibacterial agent.

 

<span style="line-height: 115% font-family: " b="" nazanin"="" font-size:="" 10pt="" mso-ascii-font-family:="" "times="" new="" roman"="" mso-hansi-font-family:="" roman""="" dir="rtl"> Materials & Methods: In this study, NPs are produced using chitosan and Tripolyphosphate (TPP) salt. Curcumin solution was loaded in chitosan NPs during their production. Next, the skins of BALB/c mice infected with staphylococcus aureus are treated by curcumin-loaded chitosan NPs for 3 days. Afterwards, in order to evaluate the antibacterial property of the nano-drug, these skin suspensions of mice are cultured in bacterial medium.

 

Results: Dynamic Light Scattering (DLS) reveals the charge of + 7 ± 2 mV and the size of 160 ± 10 nm in curcumin-loaded chitosan NPs. Moreover, Transmission electron microscopy (TEM) and atomic force microscopy (AFM) indicates a spiral shape. Therefore, the evaluation of the optical density by spectrophotometry demonstrates that 75 ± 2 % of curcumin are loaded in chitosan NPs. Bacterial culture shows that curcumin-loaded chitosan NPs significantly inhibited staphylococcus aureus growth.

Conclusion: This study demonstrates that curcumin-loaded chitosan NPs can be applied as a potent agent in treatment of bacterial skin infections.

  

Keywords: Nanoparticles, Curcumin, Chitosan, staphylococcus aureus
Full-Text [PDF 1135 kb]   (1705 Downloads)    
Type of Study: Research | Subject: Nanomedicine
Received: 2015/01/8 | Accepted: 2015/05/19 | Published: 2015/06/20
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Mofazzal Jahromi M A, Rajayi H, Al-Musawi S, Pirestani M, Fasihi Ramandi M, Ahmadi K, et al . Evaluation of Antibacterial Effect of Curcumin Loaded Chitosan Nanoparticles . J Fasa Univ Med Sci. 2015; 5 (1) :134-141
URL: http://journal.fums.ac.ir/article-1-466-en.html
Volume 5, Number 1 (Spring 2015) Back to browse issues page
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