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:: Volume 5, Number 2 (Summer 2015) ::
J Fasa Univ Med Sci 2015, 5(2): 185-195 Back to browse issues page
The Effect of Vitrification on Follicular Morphology of Ovarian Rat
Foroozan Esmaeilzadeh1, Laleh Hamedi2, Davood Mehrabani 3, Aalamtaj Bidshahri2, Nader Tanideh4
1- Assistant Professor Department of Chemistry, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran
2- Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
3- Assistant Professor Department of Biology, Islamic Azad University, Jahrom Branch , mehrabad@sums.ac.ir
4- Assistant Professor Department of Chemistry, Islamic Azad University, Marvdasht Branch
Abstract:   (4365 Views)
Background & Objective: Some efforts have been made for keeping cryopreservation of gametes and embryos safe, including new vitrification methods of the ovary. This study evaluates the effect of ethylene glycole vitrification on follicular morphology of ovarian rat.
Materials & Methods: Eighty ovaries belonging to 40 rats are divided into 2 groups. Twenty five ovaries are control group, 25 the vitrification, and30 toxicologic effects. For freezing, equilibrium solution, ethylene glycole and methyl sulfoxide are used. For defreezeing, different concentrations of saccharose and for morphological evaluation, H&E staining are undertaken. The number of healthy and atretic follicles are determined after 24 hours, 1 week and one month after vitrification.
Results: No morphological changes are observed in all follicular cells. The percent of primordial, primary, secondary, anthral and developed follicles in the vitrification group are 34.5%, 17.7%, 17.4%, 15.2% and 50.3%. In vitrification and toxicological groups, the percent of both normal and atretic follicles is 47.5% and 11.9%. These figures for the control group were 59.7% and 16.9%. In vitrification method, 91% of oocytes are viable, 81% have mitosis, and 50% enters blastocyst stage.
Conclusion: Because in vitrification of ovary in comparison with the follicles, many types of follicles in different cycles can be recovered with no morphological and structural changes, vitrification of ovary can be a safe method for cryopreservation of the oocytes
Keywords: Vitrification, Follicle, Oocyte, Ovary, Morphology, Rat
Full-Text [PDF 1122 kb]   (1178 Downloads)    
Type of Study: Research | Subject: Developmental Biology
Received: 2015/01/16 | Accepted: 2015/05/22 | Published: 2015/08/15
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Esmaeilzadeh F, Hamedi L, Mehrabani D, Bidshahri A, Tanideh N. The Effect of Vitrification on Follicular Morphology of Ovarian Rat. J Fasa Univ Med Sci. 2015; 5 (2) :185-195
URL: http://journal.fums.ac.ir/article-1-608-en.html
Volume 5, Number 2 (Summer 2015) Back to browse issues page
Journal of Fasa University of Medical Sciences
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