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:: Volume 5, Number 3 (Autumn 2015) ::
J Fasa Univ Med Sci 2015, 5(3): 368-377 Back to browse issues page
The Activity of Sirtuin 1 in MCF-7 Breast Cancer Cell Line: The Effects of Visfatin
kiarash behrouzfar1, mitra nourbakhsh 2, mohammad alaiee1
1- Department of Clinical Biochemistry, School of medicine, Tehran University of medical sciences, Tehran, Iran.
2- Department of Clinical Biochemistry, School of medicine, Iran University of medical sciences, Tehran, Iran. , : m_nourbakhsh@yahoo.com
Abstract:   (3905 Views)

Background & Objectives: Breast cancer is the most common cancer and the second leading cause of cancer deaths among women. Obesity, hormones, and growth factors are the risk factors for this kind of cancer. One of the changes observed in patients suffering from breast cancer is the elevated Visfatin or nicotinamide phosphoribosyl transferase (NAMPT) in their tumor tissues and blood. The increased activity of Visfatin and SIRT1 (Sirtuin 1) in breast cancer and many other cancers has been determined, and its value is correlated with cancer prognosis. The aim of the present study is to investigate the effects of Visfatin on SIRT1 activity in MCF-7 breast cancer cell line.

Materials & Methods: In this study, in order to investigate the effects of Visfatin on SIRT1 activity in MCF-7 cells, cells were treated after cell culture by Visfatin for 12, 24, and 48 hours. Subsequently, the cells were lysed by nuclear extraction kit, and their total protein concentrations were measured by Bradford assay. Finally, we estimated the general activity of SIRT1 by measuring the SIRT1 activity with the assay kit via spectrofluorometric device.

Results: The findings of this research show that SIRT1 activity is not significantly changed following Visfatin treatments for 12 and 24 hours. However, after 48 hour, Visfatin increases SIRT1 activity about 2 times more than control group.

Conclusion: The antiapoptotic effects of Visfatin are exerted by increasing SIRT1 activity in MCF-7 cells, and these effects happen after 24 hours. 

Keywords: Visfatin, SIRT1, Breast cancer
Full-Text [PDF 991 kb]   (1558 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2015/04/9 | Accepted: 2015/07/21 | Published: 2015/11/30
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behrouzfar K, nourbakhsh M, alaiee M. The Activity of Sirtuin 1 in MCF-7 Breast Cancer Cell Line: The Effects of Visfatin. J Fasa Univ Med Sci. 2015; 5 (3) :368-377
URL: http://journal.fums.ac.ir/article-1-787-en.html
Volume 5, Number 3 (Autumn 2015) Back to browse issues page
Journal of Fasa University of Medical Sciences
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