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:: Volume 6, Number 1 (Spring 2016) ::
J Fasa Univ Med Sci 2016, 6(1): 113-119 Back to browse issues page
Cloning and Expression of Helicobacter Pylori CagA Gene Antigenic Regions in E. coli
Mahdye Maleki1, Mohammad Reza Nassiri 2, Mojtaba Tahmoores pour31, Kiyarash Qazvini3
1- Animal Science Department, College of Agriculture, Ferdowsi university of Mashhad, Mashhad, Iran
2- Animal Science Department, College of Agriculture, Ferdowsi university of Mashhad, Mashhad, Iran , nassiryr@um.ac.ir
3- Department of Microbiology, School of Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:   (2411 Views)

Background & Objective: Helicobacter pylori, which has infected about 50 percent of the world population, is one of the most common gastrointestinal pathogens and the most prevalent cause of gastro-intestinal diseases, such as chronic gastric ulcers, gastric and duodenal ulcers, gastric cancer and lymphoma. The CagA Gene (cytotoxin-associated gene A) is a major virulence factor of this pathogenic bacterium. The aim of this study was to construct a recombinant vector carrying the antigenic regions of CagA gene and also to express this gene.
Material & methods: The major epitopes of the CagA gene were identified based on bioinformatics. Through this procedure, a gene fragment of 996 bp was isolated by PCR, inserted into a pET32a vector using the enzymatic digestion and then transformed into the E. coli strain BL2I (DE3).
Results: The results of sequencing represented the successful cloning of the CagA gene in the expression vector and introducing the accessibility to the antigen. The molecular weight of the produced recombinant protein was estimated 57 KD on an SDS-PAGE gel and the accuracy of the protein expression was verified.
Conclusions: The results of this study proved the successful cloning of the epitope area. The recombinant protein can probably be introduced as a good candidate for the production of IgY from the chickenimmunized and the control of Helicobacter pylori infection in humans. It could also be possibly used for the design of diagnostic kits and vaccines for Helicobacter pylori

Keywords: Helicobacter pylori, CagA, Recombinant protein
Full-Text [PDF 1120 kb]   (1285 Downloads)    
Type of Study: Research | Subject: Medical Biotechnology
Received: 2015/11/13 | Accepted: 2016/02/29 | Published: 2016/06/17
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Maleki M, Nassiri M R, Tahmoores pour3 M, Qazvini K. Cloning and Expression of Helicobacter Pylori CagA Gene Antigenic Regions in E. coli. J Fasa Univ Med Sci. 2016; 6 (1) :113-119
URL: http://journal.fums.ac.ir/article-1-811-en.html
Volume 6, Number 1 (Spring 2016) Back to browse issues page
Journal of Fasa University of Medical Sciences
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