Volume 6, Issue 3 (11-2016)                   JABS 2016, 6(3): 319-325 | Back to browse issues page

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Ahmadi E, Abdollahi A, Fasihi Ramandi M, Namdar N, Musavi M, Samizadeh B. Evaluation of Antibacterial Activity and Total Phenol Compounds of Punica granatum Hydro-Alcoholic Extract. JABS 2016; 6 (3) :319-325
URL: http://jabs.fums.ac.ir/article-1-853-en.html
Evaluation of Antibacterial Activity and Total Phenol Compounds of Punica granatum Hydro-Alcoholic Extract
Elahe Ahmadi1 , Abbas Abdollahi * 1, Mahdi Fasihi Ramandi2 , Najmeh Namdar1 , Mohammad Musavi1 , Babak Samizadeh1
1- Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran
2- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (10370 Views)

Background & Objectives: Punica granatum is a non-productive form of a plant and is used for the treatment of diseases in traditional medicine. In this study, we evaluate the antibacterial activity and the total phenol compounds of Punica granatum.

Materials & Methods: Disk and well diffusion methods and MIC were used to evaluate the antibacterial activity of hydro-alcoholic extract on S. aureus and E. coli compared to standard commercial antibiotic disks. Measurement of phenol compounds were performed by Seevers and Daly colorimetric methods (Folin-ciocalteu indicator).

Results: 35 and 29 mm inhibition zones in S. aureus and 22 and 17 mm inhibition zones in E. coli were shown by disk and well diffusion method, respectively. Also, 7.8 mg/ml concentration of extract showed the MIC points for two bacteria. Phenol compound of extract was 233.15±5.1 mg/g of extraction.

Conclusion: Antibacterial effect of Punica granatum compared to antibiotics indicates the strong activity against examined bacteria. Extensive antibacterial study of Punica granatum is suggested.

Keywords: Punica granatum, Antibacterial, Phenol compounds, Herbal extract
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Type of Study: Research | Subject: Pharmaceutical Plants
Received: 2016/02/14 | Accepted: 2016/05/31 | Published: 2016/12/21
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