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:: Volume 5, Number 3 (Autumn 2015) ::
J Fasa Univ Med Sci 2015, 5(3): 413-424 Back to browse issues page
Expression of Some Genes Involved in Epigenetic in Breast Cancer Cell Lines: The Effect of Quercetin
fahime mohamadian1, mehdi mahmoodi1, mohamadreza mirzae1, alireza khoshdel2, mahmood sheikh fathollahi3, nahid zeinodini4, mohamad reza hajizade 5
1- 1- Molecular medicine research center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
2- Department of Clinical Biochemical, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
3- Department of Social Medicine and Occupational Environment Research Center, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
4- Immuology of infectious diseases research center, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
5- 1- Molecular medicine research center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. , hajizadehus@yahoo.com
Abstract:   (4005 Views)

Background & Objectives: Breast cancer is one of the most common cancers among women. Incorrect pattern of gene expression involved in epigenetic including APOBEC3B, DNMT-1, and TET-1 can develop breast cancer. Quercetin is a natural flavonoid with antioxidant and anti-cancer properties that have been reported in other studies. To investigate the effect mechanism of quercetin, this study examined the effect of quercetin on the expression of genes which were referred to in two classes of breast cancer cell lines.

Materials & Methods: Cell lines including MCF-7 and MDA-MB-453 in separate boxes in the control group and the treated groups with two dosages of 50 and 100 mm of quercetin were cultured for 24 and 48 hours, respectively. RNA was extracted from the cells and then was converted to cDNA. Real-time PCR was used for APOBEC3B, DNMT_1, and TET-1 expression.

Results: The results showed that quercetin had conflicting results after 24 hours in two cell lines as there was a decrease in the gene expression of APQBEC3B and an increase in that of DNMT-1 in MCF-7 cell line. In contrast, the cell line of MDA-MB-453, APOBEC3B, and DNMT-1 gene expression increased. While the 48-hour results showed that quercetin reduced the gene expression of APOBEC3B and DNMT-1 and increased that of the TET-1 in both cell lines.

Conclusion: Due to the satisfactory effects of quercetin on breast cancer cells after 48 hours, these effects can be probably applied through epigenetic mechanisms. However, the final decision needs further investigation.

Keywords: Quercetin, Gene expression, Epigenetics, Cell lines, Breast cancer
Full-Text [PDF 833 kb]   (1359 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2015/08/6 | Accepted: 2015/09/27 | Published: 2015/12/9
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mohamadian F, mahmoodi M, mirzae M, khoshdel A, sheikh fathollahi M, zeinodini N et al . Expression of Some Genes Involved in Epigenetic in Breast Cancer Cell Lines: The Effect of Quercetin. J Fasa Univ Med Sci. 2015; 5 (3) :413-424
URL: http://journal.fums.ac.ir/article-1-881-en.html
Volume 5, Number 3 (Autumn 2015) Back to browse issues page
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