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:: Volume 6, Number 2 (Summer 2016) ::
J Fasa Univ Med Sci 2016, 6(2): 169-177 Back to browse issues page
Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375
Hossein Sazegar1, Negar Azarpira 2, Akbar Vahdati3, Mohamad hossein Karimi4, Masoud Soleimani5
1- PhD Department Of Biology, Fars Science and Research Branch, Islamc Azad University, Fars, Iran
2- MD Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3- PhD Department Of Biology, Shiraz branch, Islamc Azad University, Fars, Iran
4- PhD Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
5- PhD Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
Abstract:   (2286 Views)

Background & Objective: Type1 diabetes is characterized by autoimmune destruction of pancreatic β cells, leading to reduced insulin secretion. Differentiation of mesenchymal stem cells (MSCs) into β-like cells offers new ways of diabetes treatment. MSCs can be insulated from the human umbilical cord tissue and differentiate into insulin-producing cells.

Material & Methods: Human umbilical cord–derived stem cells (hUDSCs) were attained after birth, selected by plastic adhesion, and considered by flow cytometric analysis. For the differentiation induction, miR-375 was lentivirally over expressed in hUDSCs.

Results: Meaningful that microRNAs (miRNAs) are the key players in several stages of pancreatic Differentiation, we have presented a novel and cost-effective strategy in which the over-expression of miR-375 promotes the pancreatic differentiation in hUDSCs in the absence of any other stimulator. The major expressions of PDX1 and insulin were identified by quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) (P <0.05).

Conclusion: In conclusion, the Morphological assessment and the expression analysis of islet marker genes demonstrated that hUDSCs are able to differentiate into insulin-producing cells by transduction with lentiviral vector miR-375

Keywords: Diabetes, HUDSCs, MiR-375, Lentiviral Vector
Full-Text [PDF 1043 kb]   (774 Downloads)    
Type of Study: Research | Subject: Medical Biotechnology
Received: 2015/09/19 | Accepted: 2015/11/28 | Published: 2016/09/10
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Sazegar H, Azarpira N, Vahdati A, Karimi M H, Soleimani M. Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375. J Fasa Univ Med Sci. 2016; 6 (2) :169-177
URL: http://journal.fums.ac.ir/article-1-910-en.html
Volume 6, Number 2 (Summer 2016) Back to browse issues page
Journal of Fasa University of Medical Sciences
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