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:: Volume 6, Number 2 (Summer 2016) ::
J Fasa Univ Med Sci 2016, 6(2): 221-226 Back to browse issues page
Molecular Detection of Type 1 Fimbriae-Encoding Gene (fimH) in Uropathogenic Escherichia coli Strains Isolated from Outpatients with Urinary Tract Infection
Nemat Shams 1, Amin Jaydari2
1- Assistant professor Department of Microbiology, Faculty of Veterinary Medicine, Lorestan University, Khorram-Abad, Iran , nematshams1386@yahoo.com
2- Assistant professor Department of Microbiology, Faculty of Veterinary Medicine, Lorestan University, Khorram-Abad, Iran
Abstract:   (1816 Views)

Background & Objective: Urinary Tract Infection (UTI) is one of the most common infections in all age groups. The majority of these infections are caused by Uropathogenic Escherichia coli (UPEC) strains. Colonization, attachment to uroepithelium, and the ability of UPEC to cause symptomatic UTI is mediated by adherence factors, such as type 1 fimbriae (fimH).

The objective of this study was to determine the frequency of type 1 fimbriae-encoding gene (fimH) among uropathogenic E. coli isolates from outpatients with UTI in Khorramabad.

Materials & Methods: This laboratory study carried out on 100 uropathogenic E. coli collected in the years 2012 and 2013 from outpatients with UTI in Khorramabad. All bacterial isolates were identified by standard laboratory methods and the fimH gene presence was detected using the PCR method.

Results: The fimH gene was amplified using specific primers and showed a band about 508 bp. The FimH gene was found in 26 isolates (26%) of the UPEC strains.

Conclusion: Although results of this study showed the presence of type 1 fimbriae-encoding gene (fimH) among uropathogenic E. coli isolates from outpatients with UTI, the high prevalence of isolates that do not encode fimH (74%) require further investigation to clarify the role of the other potential virulence factors in the pathogenesis of these isolates.

Keywords: Urinary Tract Infections, Uropathogenic Escherichia coli, FimH
Full-Text [PDF 1202 kb]   (741 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2015/11/13 | Accepted: 2016/02/11 | Published: 2016/09/10
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Shams N, Jaydari A. Molecular Detection of Type 1 Fimbriae-Encoding Gene (fimH) in Uropathogenic Escherichia coli Strains Isolated from Outpatients with Urinary Tract Infection. J Fasa Univ Med Sci. 2016; 6 (2) :221-226
URL: http://journal.fums.ac.ir/article-1-933-en.html
Volume 6, Number 2 (Summer 2016) Back to browse issues page
Journal of Fasa University of Medical Sciences
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