Journal of Advanced Biomedical Sciences
JABS
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URL: http://jabs.fums.ac.ir/article-1-466-en.html
, Hajar Rajayi2 , Sharafaldin Al-Musawi3 , Majid Pirestani4 , Mahdi Fasihi Ramandi1 , Kazem Ahmadi1 , Vahid Sharifzadeh Peivasti5 , Zuhair Mohammad Hassan2 , Mahdi Kamali6 , Reza Mirnejad * 7
2- Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
3- Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
4- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
5- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
6- Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
7- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. ,
Background & Objective: Nanomedicine delivery systems are known as potent therapeutictools. In addition to possessing an effective agent of turmeric, Curcumin shows antibacterial properties as well. Curcumin is not water-soluble and it can be solved in water via nanotechnology-base methods. Chitosan is a natural and biodegradable substance that is utilized for the Production of Nanoparticles (NPs) carrying drug. In the following investigation, curcumin is loaded in chitosan NPs and ultimately, the resulting nano-drug is studied as an antibacterial agent.
<span style="line-height: 115% font-family: " b="" nazanin"="" font-size:="" 10pt="" mso-ascii-font-family:="" "times="" new="" roman"="" mso-hansi-font-family:="" roman""="" dir="rtl"> Materials & Methods: In this study, NPs are produced using chitosan and Tripolyphosphate (TPP) salt. Curcumin solution was loaded in chitosan NPs during their production. Next, the skins of BALB/c mice infected with staphylococcus aureus are treated by curcumin-loaded chitosan NPs for 3 days. Afterwards, in order to evaluate the antibacterial property of the nano-drug, these skin suspensions of mice are cultured in bacterial medium.
Results: Dynamic Light Scattering (DLS) reveals the charge of + 7 ± 2 mV and the size of 160 ± 10 nm in curcumin-loaded chitosan NPs. Moreover, Transmission electron microscopy (TEM) and atomic force microscopy (AFM) indicates a spiral shape. Therefore, the evaluation of the optical density by spectrophotometry demonstrates that 75 ± 2 % of curcumin are loaded in chitosan NPs. Bacterial culture shows that curcumin-loaded chitosan NPs significantly inhibited staphylococcus aureus growth.
Conclusion: This study demonstrates that curcumin-loaded chitosan NPs can be applied as a potent agent in treatment of bacterial skin infections.
Received: 2015/01/8 | Accepted: 2015/05/19 | Published: 2015/06/20
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This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)