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:: Volume 9, Issue 3 (Autumn 2019) ::
J Fasa Univ Med Sci 2019, 9(3): 1665-1674 Back to browse issues page
Quantitative Comparison of CK19 Gene Expression Between Normal Iranian Women and Ovarian Cancer Blood Sample by Real Time PCR
Seiedeh Razieh Sadat Shirazi1 , Ardeshir Hesampour 2
1- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , a.hesampour@gmail.com
Abstract:   (577 Views)
Background & Objectives: Ovarian cancer is the fifth leading cause of cancer deaths in Iran and the United States women population. Late detection of illness could lead to death of the patient and has a lot of cost expenses to economy, so early diagnosis of pain and costs imposed for patients will be reduced.CK19 has the potential for early diagnosis of ovarian cancer. In this study, the potential for gene expression of CK19 as a molecular biomarker for the diagnosis of ovarian cancer in the circulatory system was investigated using Real Time PCR technique, so that it could be further investigated evaluated about the mechanism and effect of this gene, the title is a good target for treating ovarian cancer.
Material & Methods: In this case-control study, 50 blood samples from women with ovarian cancer and 50 normal control blood samples from patients referred to Imam Khomeini Hospital were reviewed. After collecting samples, extraction Total RNA from frozen ovarian epithelial blood samples, cDNA was performed from the cDNA. Finally, the expression of the CK19 gene in 50 patient blood samples and 50 normal blood samples was investigated using qRT-PCR technique.
Results: We determined that CK19 was significantly up-regulated in ovarian cancer, compared to normal blood sample. Further investigations revealed that this overexpression is elevated by increasing the malignancy stage.
Conclusion: This test showed a significant increase CK19 in patients, expression of CK19 was quantitatively measured and expression of CK19 was reported in the serum of positive patients. Also according to grade the patient was different in the serum of patients with CK19 and the higher the patient progressed the higher the expression.
 
Keywords: Ovarian cancer, CK19 Marker, Real Time PCR
Full-Text [PDF 784 kb]   (99 Downloads)    
Type of Study: Research | Subject: Cellular-Molecular Biology
Received: 2018/12/4 | Accepted: 2019/06/3 | Published: 2019/12/10
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Sadat Shirazi S R, Hesampour A. Quantitative Comparison of CK19 Gene Expression Between Normal Iranian Women and Ovarian Cancer Blood Sample by Real Time PCR. J Fasa Univ Med Sci. 2019; 9 (3) :1665-1674
URL: http://journal.fums.ac.ir/article-1-1612-en.html


Volume 9, Issue 3 (Autumn 2019) Back to browse issues page
Journal of Fasa University of Medical Sciences
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